Introduction to genetic circuits in natural systems; engineering principles in biology; BioBricks and standardization of biological components; numerical methods for systems analysis and design; fabrication of genetic systems in theory and practice; transformation and characterization; examples of engineered systems; hands-on experiments. Principles and applications of biotechnology; introduction to key enabling technologies; genetic circuits in natural systems; engineering principles in biology; BioBricks and standardization of biological components; numerical methods for systems analysis and design; fabrication of genetic systems in theory and practice; transformation and characterization; examples of engineered systems.