Establishment of patient-derived iPSCs from fibroblast using integration-free and xeno-free reprogramming methods

Induced pluripotent stem cells (iPSCs) have indefinite proliferation capability and can differentiate into different cell types. Somatic cells can easily reverse back to pluripotent state through the activation of reprogramming factors. iPSCs-derived organoids are widely used in regenerative medicine and disease modeling. Here, we performed an integration-free reprogramming method and xeno-free culture condition to reprogram patient fibroblasts and showed the resulting iPSCs are genuine pluripotent stem cells through detection of pluripotency markers and embryoid body formation. We also investigated exogenous DNA existence to confirm our iPSCs are footprint-free. We reprogrammed 14 fibroblasts cell lines and made a huge bio-bank for neural disease-related iPSCs. These iPSCs will send to Heine University Düsseldorf (HHU) to make brain organoids, which is helpful to study the patient-specific mechanism of the disease.