A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells

Yihua Loo; Yun S. Chan; Iwona Szczerbinska; Bobby C. P. Tan; Andrew C.A Wan; Huck Hui Ng; Charlotte Hauser

doi:10.1021/acsabm.8b00686

A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells

Yihua Loo, Yun S. Chan, Iwona Szczerbinska, Bobby C. P. Tan, Andrew C.A Wan, Huck Hui Ng, Charlotte Hauser

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.

Original language	English (US)
Pages (from-to)	1406-1412
Number of pages	7
Journal	ACS Applied Bio Materials
Volume	2
Issue number	4
DOIs	https://doi.org/10.1021/acsabm.8b00686
State	Published - Mar 2019

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10.1021/acsabm.8b00686

https://repository.kaust.edu.sa/bitstream/10754/631541/1/acsabm.8b00686.pdf

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@article{dabb6103ba2944a0b3d6a0b1781979a5,

title = "A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells",

abstract = "Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.",

author = "Yihua Loo and Chan, {Yun S.} and Iwona Szczerbinska and Tan, {Bobby C. P.} and Wan, {Andrew C.A} and Ng, {Huck Hui} and Charlotte Hauser",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the Institute of Bioengineering and Nanotechnology, Genome Institute of Singapore, and Biomedical Research Council (Agency for Science, Technology and Research, Singapore), as well as by grant funding to Charlotte A. E. Hauser from the Agency for Science, Technology and Research (A*STAR) Joint Council Office (JCO) Grant 1231AFG023. Yihua Loo was also supported by the A*STAR BMRC YIG Grant 14/1/07/51/011.",

year = "2019",

month = mar,

doi = "10.1021/acsabm.8b00686",

language = "English (US)",

volume = "2",

pages = "1406--1412",

journal = "ACS Applied Bio Materials",

issn = "2576-6422",

publisher = "American Chemical Society",

number = "4",

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T1 - A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells

AU - Loo, Yihua

AU - Chan, Yun S.

AU - Szczerbinska, Iwona

AU - Tan, Bobby C. P.

AU - Wan, Andrew C.A

AU - Ng, Huck Hui

AU - Hauser, Charlotte

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the Institute of Bioengineering and Nanotechnology, Genome Institute of Singapore, and Biomedical Research Council (Agency for Science, Technology and Research, Singapore), as well as by grant funding to Charlotte A. E. Hauser from the Agency for Science, Technology and Research (A*STAR) Joint Council Office (JCO) Grant 1231AFG023. Yihua Loo was also supported by the A*STAR BMRC YIG Grant 14/1/07/51/011.

PY - 2019/3

Y1 - 2019/3

N2 - Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.

AB - Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.

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DO - 10.1021/acsabm.8b00686

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JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

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A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells

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