A comparative study of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) versus SCX-IMAC-based methods for phosphopeptide isolation/enrichment

Chee Sian Gan; Tiannan Guo; Huoming Zhang; Sal Kiang Lim; Siu Kwan Sze

doi:10.1021/pr800473j

A comparative study of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) versus SCX-IMAC-based methods for phosphopeptide isolation/enrichment

Chee Sian Gan, Tiannan Guo, Huoming Zhang, Sal Kiang Lim, Siu Kwan Sze

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.

Original language	English (US)
Pages (from-to)	4869-4877
Number of pages	9
Journal	Journal of Proteome Research
Volume	7
Issue number	11
DOIs	https://doi.org/10.1021/pr800473j
State	Published - Nov 2008
Externally published	Yes

Keywords

A431 carcinoma cells
EGFR signaling
ERLIC
IMAC
Phosphopeptide enrichment
SCX
WAX

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr800473j

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title = "A comparative study of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) versus SCX-IMAC-based methods for phosphopeptide isolation/enrichment",

abstract = "Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.",

keywords = "A431 carcinoma cells, EGFR signaling, ERLIC, IMAC, Phosphopeptide enrichment, SCX, WAX",

author = "Gan, {Chee Sian} and Tiannan Guo and Huoming Zhang and Lim, {Sal Kiang} and Sze, {Siu Kwan}",

year = "2008",

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language = "English (US)",

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AU - Guo, Tiannan

AU - Zhang, Huoming

AU - Lim, Sal Kiang

AU - Sze, Siu Kwan

PY - 2008/11

Y1 - 2008/11

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AB - Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.

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KW - EGFR signaling

KW - ERLIC

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A comparative study of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) versus SCX-IMAC-based methods for phosphopeptide isolation/enrichment

Abstract

Keywords

ASJC Scopus subject areas

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