A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains

Takeshi Itoh; Toshitsugu Okayama; Hiroyuki Hashimoto; Jun Ichi Takeda; Ronald W. Davis; Hirotada Mori; Takashi Gojobori

doi:10.1016/S0014-5793(99)00481-0

A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains

Takeshi Itoh, Toshitsugu Okayama, Hiroyuki Hashimoto, Jun Ichi Takeda, Ronald W. Davis, Hirotada Mori, Takashi Gojobori^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli substrains. Given that the two substrains separated about 40 years ago, the rate of nucleotide changes was estimated to be less than 10^-7 per site per year. This rate was supported by a further comparison between partial genome sequences of E. coli and Shigella flexneri. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	72-76
Number of pages	5
Journal	FEBS Letters
Volume	450
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(99)00481-0
State	Published - Apr 30 1999
Externally published	Yes

Keywords

Complete genome
Escherichia coli
Genome project
Insertion sequence
Rate of nucleotide change
Rate of nucleotide substitution

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00481-0

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title = "A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains",

abstract = "Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli substrains. Given that the two substrains separated about 40 years ago, the rate of nucleotide changes was estimated to be less than 10-7 per site per year. This rate was supported by a further comparison between partial genome sequences of E. coli and Shigella flexneri. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Complete genome, Escherichia coli, Genome project, Insertion sequence, Rate of nucleotide change, Rate of nucleotide substitution",

author = "Takeshi Itoh and Toshitsugu Okayama and Hiroyuki Hashimoto and Takeda, {Jun Ichi} and Davis, {Ronald W.} and Hirotada Mori and Takashi Gojobori",

note = "Funding Information: We appreciate our colleagues for helpful discussion. We would like to thank S. Gaudieri and R. Chapman for proof reading of the manuscript. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan.",

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T1 - A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains

AU - Itoh, Takeshi

AU - Okayama, Toshitsugu

AU - Hashimoto, Hiroyuki

AU - Takeda, Jun Ichi

AU - Davis, Ronald W.

AU - Mori, Hirotada

AU - Gojobori, Takashi

N1 - Funding Information: We appreciate our colleagues for helpful discussion. We would like to thank S. Gaudieri and R. Chapman for proof reading of the manuscript. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 1999/4/30

Y1 - 1999/4/30

N2 - Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli substrains. Given that the two substrains separated about 40 years ago, the rate of nucleotide changes was estimated to be less than 10-7 per site per year. This rate was supported by a further comparison between partial genome sequences of E. coli and Shigella flexneri. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli substrains. Given that the two substrains separated about 40 years ago, the rate of nucleotide changes was estimated to be less than 10-7 per site per year. This rate was supported by a further comparison between partial genome sequences of E. coli and Shigella flexneri. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Complete genome

KW - Escherichia coli

KW - Genome project

KW - Insertion sequence

KW - Rate of nucleotide change

KW - Rate of nucleotide substitution

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U2 - 10.1016/S0014-5793(99)00481-0

DO - 10.1016/S0014-5793(99)00481-0

M3 - Article

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AN - SCOPUS:0033007991

SN - 0014-5793

VL - 450

SP - 72

EP - 76

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains

Abstract

Keywords

ASJC Scopus subject areas

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