A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants.

Jianing Mi; Kunpeng Jia; Aparna Balakrishna; Salim Al-Babili

doi:10.1007/978-1-4939-9952-1_13

A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants.

Jianing Mi, Kunpeng Jia, Aparna Balakrishna, Salim Al-Babili

Research output: Chapter in Book/Report/Conference proceeding › Chapter

5 Scopus citations

Abstract

We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.

Original language	English (US)
Title of host publication	Methods in molecular biology (Clifton, N.J.)
Publisher	Springer US
Pages	177-188
Number of pages	12
ISBN (Print)	9781493999514
DOIs	https://doi.org/10.1007/978-1-4939-9952-1_13
State	Published - Nov 20 2019

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10.1007/978-1-4939-9952-1_13

https://repository.kaust.edu.sa/bitstream/10754/660431/1/Mi%20et%20al.%202019-accepted.pdf

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title = "A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants.",

abstract = "We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.",

author = "Jianing Mi and Kunpeng Jia and Aparna Balakrishna and Salim Al-Babili",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): CRG4 Acknowledgements: This work was supported by base line funding and a Competitive Research Grant (CRG4) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).",

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doi = "10.1007/978-1-4939-9952-1_13",

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AU - Mi, Jianing

AU - Jia, Kunpeng

AU - Balakrishna, Aparna

AU - Al-Babili, Salim

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): CRG4 Acknowledgements: This work was supported by base line funding and a Competitive Research Grant (CRG4) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).

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Y1 - 2019/11/20

N2 - We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.

AB - We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.

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BT - Methods in molecular biology (Clifton, N.J.)

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ER -

A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants.

Abstract

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