TY - GEN
T1 - A new equivalent circuit model for micro electroporation systems
AU - Shagoshtasbi, Hooman
AU - Lee, Yi-Kuen
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: Department of Mechanical Engineering, HKUST, Clear Water Bay, Kowloon, Hong Kong
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2011/2
Y1 - 2011/2
N2 - Electroporation (EP) is a unique biotechnique in which intense electric pulses are applied on the cell membrane to temporarily generate nanoscale electropores and to increase the membrane permeability for the delivery of exogenous biomolecules or drugs. We propose a new equivalent circuit model with 8 electric components to predict the electrodynamic response of a micro EP system. As the permeability of the cell membrane increases, the membrane resistance decreases. The numerical simulations of the transmembrane current responses to different applied voltages (1∼6V) are consistent with the experimental results using HeLa cells. Besides, the transmembrane voltage as a function of applied voltages is determined as well. These transmembrane current and voltage responses can be extremely useful for the design of new generation of micro EP systems for transfection of large DNA molecules in the future. © 2011 IEEE.
AB - Electroporation (EP) is a unique biotechnique in which intense electric pulses are applied on the cell membrane to temporarily generate nanoscale electropores and to increase the membrane permeability for the delivery of exogenous biomolecules or drugs. We propose a new equivalent circuit model with 8 electric components to predict the electrodynamic response of a micro EP system. As the permeability of the cell membrane increases, the membrane resistance decreases. The numerical simulations of the transmembrane current responses to different applied voltages (1∼6V) are consistent with the experimental results using HeLa cells. Besides, the transmembrane voltage as a function of applied voltages is determined as well. These transmembrane current and voltage responses can be extremely useful for the design of new generation of micro EP systems for transfection of large DNA molecules in the future. © 2011 IEEE.
UR - http://hdl.handle.net/10754/597337
UR - http://ieeexplore.ieee.org/document/6017442/
UR - http://www.scopus.com/inward/record.url?scp=80053333682&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2011.6017442
DO - 10.1109/NEMS.2011.6017442
M3 - Conference contribution
SN - 9781612847757
SP - 662
EP - 665
BT - 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
PB - Institute of Electrical and Electronics Engineers (IEEE)
ER -