TY - JOUR
T1 - A Passive Microfluidic Device for Chemotaxis Studies
AU - Coluccio, Maria Laura
AU - D’Attimo, Maria Antonia
AU - Cristiani, Costanza Maria
AU - Candeloro, Patrizio
AU - Parrotta, Elvira
AU - Dattola, Elisabetta
AU - Guzzi, Francesco
AU - Cuda, Giovanni
AU - Lamanna, Ernesto
AU - Carbone, Ennio
AU - Krühne, Ulrich
AU - Di Fabrizio, Enzo M.
AU - Perozziello, Gerardo
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the project for young researchers financed from the Italian Ministry of Health “High throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University “Magna Graecia” of Catanzaro.
PY - 2019/8/20
Y1 - 2019/8/20
N2 - This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.
AB - This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.
UR - http://hdl.handle.net/10754/656611
UR - https://www.mdpi.com/2072-666X/10/8/551
UR - http://www.scopus.com/inward/record.url?scp=85070973798&partnerID=8YFLogxK
U2 - 10.3390/mi10080551
DO - 10.3390/mi10080551
M3 - Article
C2 - 31434220
SN - 2072-666X
VL - 10
SP - 551
JO - Micromachines
JF - Micromachines
IS - 8
ER -