A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins

Bettina M. Lengsfeld; Kayla N. Berry; Sharmistha Ghosh; Masateru Takahashi; Nicole J. Francis

doi:10.1038/srep00661

A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins

Bettina M. Lengsfeld, Kayla N. Berry, Sharmistha Ghosh, Masateru Takahashi, Nicole J. Francis

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.

Original language	English (US)
Journal	Scientific Reports
Volume	2
Issue number	1
DOIs	https://doi.org/10.1038/srep00661
State	Published - Sep 17 2012

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https://repository.kaust.edu.sa/bitstream/10754/325371/1/Article-Scientific-A_Polycomb-2012.pdf

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abstract = "Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.",

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AU - Ghosh, Sharmistha

AU - Takahashi, Masateru

AU - Francis, Nicole J.

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N2 - Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.

AB - Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.

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A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins

Abstract

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