A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Michael J. Edel; Cristina Menchon; Jose Miguel Andres Vaquero; Juan Carlos Izpisua Belmonte

doi:10.1186/1478-811X-9-8

A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Michael J. Edel, Cristina Menchon, Jose Miguel Andres Vaquero, Juan Carlos Izpisua Belmonte^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.

Original language	English (US)
Article number	8
Journal	Cell Communication and Signaling
Volume	9
DOIs	https://doi.org/10.1186/1478-811X-9-8
State	Published - 2011
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/1478-811X-9-8

Cite this

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title = "A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells",

abstract = "Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.",

author = "Edel, {Michael J.} and Cristina Menchon and Vaquero, {Jose Miguel Andres} and {Izpisua Belmonte}, {Juan Carlos}",

note = "Funding Information: The authors are indebted to Meritxell Carri{\'o} for expert assistance with cell culture techniques. Cristina Menchon is partly supported by a pre-doctoral grant from the Spanish Ministry (AP-2004-3523). This work was partially supported by grants from the Fondo de Investigaciones Sanitarias, Insituto Carlos III (P1071209), MICINN, Fundacion Cellex, CIBER, Sanofi-Aventis and the G. Harold and Leila Y. Mathers Charitable Foundation.",

year = "2011",

doi = "10.1186/1478-811X-9-8",

language = "English (US)",

volume = "9",

journal = "Cell Communication and Signaling",

issn = "1478-811X",

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TY - JOUR

T1 - A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

AU - Edel, Michael J.

AU - Menchon, Cristina

AU - Vaquero, Jose Miguel Andres

AU - Izpisua Belmonte, Juan Carlos

N1 - Funding Information: The authors are indebted to Meritxell Carrió for expert assistance with cell culture techniques. Cristina Menchon is partly supported by a pre-doctoral grant from the Spanish Ministry (AP-2004-3523). This work was partially supported by grants from the Fondo de Investigaciones Sanitarias, Insituto Carlos III (P1071209), MICINN, Fundacion Cellex, CIBER, Sanofi-Aventis and the G. Harold and Leila Y. Mathers Charitable Foundation.

PY - 2011

Y1 - 2011

N2 - Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.

AB - Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.

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U2 - 10.1186/1478-811X-9-8

DO - 10.1186/1478-811X-9-8

M3 - Article

C2 - 21481269

AN - SCOPUS:79953779299

SN - 1478-811X

VL - 9

JO - Cell Communication and Signaling

JF - Cell Communication and Signaling

M1 - 8

ER -

A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Abstract

ASJC Scopus subject areas

UN SDGs

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