A rapid procedure for the isolation of C0t-1 DNA from plants

Michael S. Zwick; Robert E. Hanson; Thomas D. McKnight; M. Nurul Islam-Faridi; David M. Stelly; Rod A. Wing; H. James Price

doi:10.1139/g97-020

A rapid procedure for the isolation of C0t-1 DNA from plants

Michael S. Zwick, Robert E. Hanson, Thomas D. McKnight, M. Nurul Islam-Faridi, David M. Stelly, Rod A. Wing, H. James Price

Research output: Contribution to journal › Article › peer-review

208 Scopus citations

Abstract

In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or 'blocking' of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.

Original language	English (US)
Journal	Genome
Volume	40
Issue number	1
DOIs	https://doi.org/10.1139/g97-020
State	Published - Jan 1 1997
Externally published	Yes

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title = "A rapid procedure for the isolation of C0t-1 DNA from plants",

abstract = "In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or 'blocking' of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.",

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AU - Zwick, Michael S.

AU - Hanson, Robert E.

AU - McKnight, Thomas D.

AU - Islam-Faridi, M. Nurul

AU - Stelly, David M.

AU - Wing, Rod A.

AU - Price, H. James

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N2 - In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or 'blocking' of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.

AB - In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or 'blocking' of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.

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A rapid procedure for the isolation of C0t-1 DNA from plants

Abstract

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