A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

Jianping Zhang; Timothy C. Hewitt; Willem H.P. Boshoff; Ian Dundas; Narayana Upadhyaya; Jianbo Li; Mehran Patpour; Sutha Chandramohan; Zacharias A. Pretorius; Mogens Hovmøller; Wendelin Schnippenkoetter; Robert F. Park; Rohit Mago; Sambasivam Periyannan; Dhara Bhatt; Sami Hoxha; Soma Chakraborty; Ming Luo; Peter Dodds; Burkhard Steuernagel; Brande B.H. Wulff; Michael Ayliffe; Robert A. McIntosh; Peng Zhang; Evans S. Lagudah

doi:10.1038/s41467-021-23738-0

A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

Jianping Zhang, Timothy C. Hewitt, Willem H.P. Boshoff, Ian Dundas, Narayana Upadhyaya, Jianbo Li, Mehran Patpour, Sutha Chandramohan, Zacharias A. Pretorius, Mogens Hovmøller, Wendelin Schnippenkoetter, Robert F. Park, Rohit Mago, Sambasivam Periyannan, Dhara Bhatt, Sami Hoxha, Soma Chakraborty, Ming Luo, Peter Dodds, Burkhard SteuernagelBrande B.H. Wulff, Michael Ayliffe, Robert A. McIntosh, Peng Zhang, Evans S. Lagudah

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.

Original language	English (US)
Journal	Nature Communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-23738-0
State	Published - Dec 1 2021
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Chemistry
General Physics and Astronomy

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Zhang, J., Hewitt, T. C., Boshoff, W. H. P., Dundas, I., Upadhyaya, N., Li, J., Patpour, M., Chandramohan, S., Pretorius, Z. A., Hovmøller, M., Schnippenkoetter, W., Park, R. F., Mago, R., Periyannan, S., Bhatt, D., Hoxha, S., Chakraborty, S., Luo, M., Dodds, P., ... Lagudah, E. S. (2021). A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-23738-0

@article{117d3ef06fca4f129d52f667a604fd44,

title = "A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes",

abstract = "The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.",

author = "Jianping Zhang and Hewitt, {Timothy C.} and Boshoff, {Willem H.P.} and Ian Dundas and Narayana Upadhyaya and Jianbo Li and Mehran Patpour and Sutha Chandramohan and Pretorius, {Zacharias A.} and Mogens Hovm{\o}ller and Wendelin Schnippenkoetter and Park, {Robert F.} and Rohit Mago and Sambasivam Periyannan and Dhara Bhatt and Sami Hoxha and Soma Chakraborty and Ming Luo and Peter Dodds and Burkhard Steuernagel and Wulff, {Brande B.H.} and Michael Ayliffe and McIntosh, {Robert A.} and Peng Zhang and Lagudah, {Evans S.}",

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year = "2021",

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day = "1",

doi = "10.1038/s41467-021-23738-0",

language = "English (US)",

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Zhang, J, Hewitt, TC, Boshoff, WHP, Dundas, I, Upadhyaya, N, Li, J, Patpour, M, Chandramohan, S, Pretorius, ZA, Hovmøller, M, Schnippenkoetter, W, Park, RF, Mago, R, Periyannan, S, Bhatt, D, Hoxha, S, Chakraborty, S, Luo, M, Dodds, P, Steuernagel, B, Wulff, BBH, Ayliffe, M, McIntosh, RA, Zhang, P & Lagudah, ES 2021, 'A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes', Nature Communications, vol. 12, no. 1. https://doi.org/10.1038/s41467-021-23738-0

TY - JOUR

T1 - A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

AU - Zhang, Jianping

AU - Hewitt, Timothy C.

AU - Boshoff, Willem H.P.

AU - Dundas, Ian

AU - Upadhyaya, Narayana

AU - Li, Jianbo

AU - Patpour, Mehran

AU - Chandramohan, Sutha

AU - Pretorius, Zacharias A.

AU - Hovmøller, Mogens

AU - Schnippenkoetter, Wendelin

AU - Park, Robert F.

AU - Mago, Rohit

AU - Periyannan, Sambasivam

AU - Bhatt, Dhara

AU - Hoxha, Sami

AU - Chakraborty, Soma

AU - Luo, Ming

AU - Dodds, Peter

AU - Steuernagel, Burkhard

AU - Wulff, Brande B.H.

AU - Ayliffe, Michael

AU - McIntosh, Robert A.

AU - Zhang, Peng

AU - Lagudah, Evans S.

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-20

PY - 2021/12/1

Y1 - 2021/12/1

N2 - The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.

AB - The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.

UR - https://www.nature.com/articles/s41467-021-23738-0

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U2 - 10.1038/s41467-021-23738-0

DO - 10.1038/s41467-021-23738-0

M3 - Article

SN - 2041-1723

VL - 12

JO - Nature Communications

JF - Nature Communications

IS - 1

ER -

A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

Abstract

ASJC Scopus subject areas

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