A SCARECROW-RETINOBLASTOMA Protein Network Controls Protective Quiescence in the Arabidopsis Root Stem Cell Organizer

Alfredo Cruz-Ramírez; Sara Díaz-Triviño; Guy Wachsman; Yujuan Du; Mario Arteága-Vázquez; Hongtao Zhang; Rene Benjamins; Ikram Blilou; Anne B. Neef; Vicki Chandler; Ben Scheres

doi:10.1371/journal.pbio.1001724

A SCARECROW-RETINOBLASTOMA Protein Network Controls Protective Quiescence in the Arabidopsis Root Stem Cell Organizer

Alfredo Cruz-Ramírez, Sara Díaz-Triviño, Guy Wachsman, Yujuan Du, Mario Arteága-Vázquez, Hongtao Zhang, Rene Benjamins, Ikram Blilou, Anne B. Neef, Vicki Chandler, Ben Scheres^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

131 Scopus citations

Abstract

Quiescent long-term somatic stem cells reside in plant and animal stem cell niches. Within the Arabidopsis root stem cell population, the Quiescent Centre (QC), which contains slowly dividing cells, maintains surrounding short-term stem cells and may act as a long-term reservoir for stem cells. The RETINOBLASTOMA-RELATED (RBR) protein cell-autonomously reinforces mitotic quiescence in the QC. RBR interacts with the stem cell transcription factor SCARECROW (SCR) through an LxCxE motif. Disruption of this interaction by point mutation in SCR or RBR promotes asymmetric divisions in the QC that renew short-term stem cells. Analysis of the in vivo role of quiescence in the root stem cell niche reveals that slow cycling within the QC is not needed for structural integrity of the niche but allows the growing root to cope with DNA damage.

Original language	English (US)
Article number	e1001724
Journal	PLOS Biology
Volume	11
Issue number	11
DOIs	https://doi.org/10.1371/journal.pbio.1001724
State	Published - Nov 2013
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Agricultural and Biological Sciences

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Cruz-Ramírez, A., Díaz-Triviño, S., Wachsman, G., Du, Y., Arteága-Vázquez, M., Zhang, H., Benjamins, R., Blilou, I., Neef, A. B., Chandler, V., & Scheres, B. (2013). A SCARECROW-RETINOBLASTOMA Protein Network Controls Protective Quiescence in the Arabidopsis Root Stem Cell Organizer. PLOS Biology, 11(11), Article e1001724. https://doi.org/10.1371/journal.pbio.1001724

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abstract = "Quiescent long-term somatic stem cells reside in plant and animal stem cell niches. Within the Arabidopsis root stem cell population, the Quiescent Centre (QC), which contains slowly dividing cells, maintains surrounding short-term stem cells and may act as a long-term reservoir for stem cells. The RETINOBLASTOMA-RELATED (RBR) protein cell-autonomously reinforces mitotic quiescence in the QC. RBR interacts with the stem cell transcription factor SCARECROW (SCR) through an LxCxE motif. Disruption of this interaction by point mutation in SCR or RBR promotes asymmetric divisions in the QC that renew short-term stem cells. Analysis of the in vivo role of quiescence in the root stem cell niche reveals that slow cycling within the QC is not needed for structural integrity of the niche but allows the growing root to cope with DNA damage.",

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AU - Wachsman, Guy

AU - Du, Yujuan

AU - Arteága-Vázquez, Mario

AU - Zhang, Hongtao

AU - Benjamins, Rene

AU - Blilou, Ikram

AU - Neef, Anne B.

AU - Chandler, Vicki

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A SCARECROW-RETINOBLASTOMA Protein Network Controls Protective Quiescence in the Arabidopsis Root Stem Cell Organizer

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ASJC Scopus subject areas

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