A Sensitive Ratiometric Long-Wavelength Fluorescent Probe for Selective Determination of Cysteine/Homocysteine

Kesavan Manibalan, Sin Ming Chen, Veerappan Mani, Tsung Tao Huang, Sheng Tung Huang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


The development of sensitive fluorescence probes to detect biothiols such as cysteine and homocysteine has attracted great attention in recent times. Herein, we described the design and synthesis of coumarin based long-wavelength fluorescence probe, Bromo-2-benzothiazolyl-3-cyano-7-hydroxy coumarin (BBCH, 2) for selective detections of cysteine and homocysteine. The probe is rationally designed in such a way that both sulfhydryl and adjacent amino groups of thiols are involved in sensing process. Only cysteine/homocysteine able to react with BBCH to release fluorescence reporter (BCH, 1); while, glutathione and other amino acids unable to react with BBCH due to the absence of adjacent amino groups. In presence of cysteine, the color of BBCH is turns from colorless to red and thus BBCH is a naked eye fluorescence indicator for cysteine. Besides, BBCH can discriminate cysteine and homocysteine based on color changes and different reaction rates. The described sensing platform showed good sensing performances to detect cysteine and homocysteine with detection limits of 0.87 and 0.19 μM, respectively. Practical applicability was verified in biological and pharmaceutical samples.
Original languageEnglish (US)
Pages (from-to)1489-1495
Number of pages7
JournalJournal of Fluorescence
Issue number4
StatePublished - Jul 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry


Dive into the research topics of 'A Sensitive Ratiometric Long-Wavelength Fluorescent Probe for Selective Determination of Cysteine/Homocysteine'. Together they form a unique fingerprint.

Cite this