A simple system for in-droplet incubation and quantification of agglutination assays

D. Castro*, R. Kodzius, I. G. Foulds

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in microchannels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (∼2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1598-1600
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Agglutination assays
  • Droplets
  • Image processing
  • Microfluidic

ASJC Scopus subject areas

  • Bioengineering

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