A simple system for in-droplet incubation and quantification of agglutination assays

Daniela Castro; Rimantas Kodzius; Ian Grant Foulds

A simple system for in-droplet incubation and quantification of agglutination assays

Daniela Castro, Rimantas Kodzius, Ian Grant Foulds

Computer, Electrical and Mathematical Sciences and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Scopus citations

Abstract

This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in microchannels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (∼2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.

Original language	English (US)
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Publisher	Chemical and Biological Microsystems Society
ISBN (Print)	9781632666246
State	Published - Jan 1 2013

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https://repository.kaust.edu.sa/bitstream/10754/580854/1/A%20simple%20system%20for%20in-droplet%20incubation%20and%20quantification%20of%20agglutination%20assays%20535_0985.pdf

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Castro, D., Kodzius, R., & Foulds, I. G. (2013). A simple system for in-droplet incubation and quantification of agglutination assays. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 Chemical and Biological Microsystems Society. https://repository.kaust.edu.sa/bitstream/10754/580854/1/A%20simple%20system%20for%20in-droplet%20incubation%20and%20quantification%20of%20agglutination%20assays%20535_0985.pdf

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title = "A simple system for in-droplet incubation and quantification of agglutination assays",

abstract = "This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in microchannels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (∼2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.",

author = "Daniela Castro and Rimantas Kodzius and Foulds, {Ian Grant}",

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year = "2013",

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Castro, D, Kodzius, R & Foulds, IG 2013, A simple system for in-droplet incubation and quantification of agglutination assays. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. <https://repository.kaust.edu.sa/bitstream/10754/580854/1/A%20simple%20system%20for%20in-droplet%20incubation%20and%20quantification%20of%20agglutination%20assays%20535_0985.pdf>

A simple system for in-droplet incubation and quantification of agglutination assays. / Castro, Daniela; Kodzius, Rimantas; Foulds, Ian Grant.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A simple system for in-droplet incubation and quantification of agglutination assays

AU - Castro, Daniela

AU - Kodzius, Rimantas

AU - Foulds, Ian Grant

N1 - KAUST Repository Item: Exported on 2020-04-23

PY - 2013/1/1

Y1 - 2013/1/1

N2 - This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in microchannels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (∼2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.

AB - This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in microchannels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (∼2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.

UR - http://hdl.handle.net/10754/580854

UR - http://www.microtas2013.org/

M3 - Conference contribution

AN - SCOPUS:84907374026

SN - 9781632666246

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

ER -

A simple system for in-droplet incubation and quantification of agglutination assays

Abstract

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