TY - GEN
T1 - A study of the incubation of microbead agglutination assays in a microfluidic system
AU - Castro, David
AU - Conchouso Gonzalez, David
AU - Carreno, Armando Arpys Arevalo
AU - Foulds, Ian G.
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2016/12/19
Y1 - 2016/12/19
N2 - This work reports on a quantitative study of the incubation of a microbead-based agglutination assay inside a microfluidic system. In this system, a droplet (1.25µL) consisting of a mixture of functionalized microbeads and analyte is flowed through a 0.51mm internal diameter silicone tube. Hydrodynamic forces alone produce a very efficient mixing of the beads within the droplet. We tested the agglutination at different speeds and show a robust response at the higher range of speeds (150 – 200µL/min), while also reaching a completion in the agglutination process. At these velocities, a length of 180cm is shown to be sufficient to confidently measure the agglutination assay, which takes between 2.5 – 3 minutes. This high throughput quantification method has the potential of accelerating the measurements of various types of biomarkers, which can greatly benefit the fields of biology and medicine.
AB - This work reports on a quantitative study of the incubation of a microbead-based agglutination assay inside a microfluidic system. In this system, a droplet (1.25µL) consisting of a mixture of functionalized microbeads and analyte is flowed through a 0.51mm internal diameter silicone tube. Hydrodynamic forces alone produce a very efficient mixing of the beads within the droplet. We tested the agglutination at different speeds and show a robust response at the higher range of speeds (150 – 200µL/min), while also reaching a completion in the agglutination process. At these velocities, a length of 180cm is shown to be sufficient to confidently measure the agglutination assay, which takes between 2.5 – 3 minutes. This high throughput quantification method has the potential of accelerating the measurements of various types of biomarkers, which can greatly benefit the fields of biology and medicine.
UR - http://hdl.handle.net/10754/622509
UR - http://ieeexplore.ieee.org/document/7758266/
UR - http://www.scopus.com/inward/record.url?scp=85007275332&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2016.7758266
DO - 10.1109/NEMS.2016.7758266
M3 - Conference contribution
SN - 9781509019472
SP - 354
EP - 357
BT - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
PB - Institute of Electrical and Electronics Engineers (IEEE)
ER -