TY - JOUR
T1 - A two-stage biological gas to liquid transfer process to convert carbon dioxide into bioplastic
AU - Al Rowaihi, Israa
AU - Kick, Benjamin
AU - Grötzinger, Stefan W.
AU - Burger, Christian
AU - Karan, Ram
AU - Weuster-Botz, Dirk
AU - Eppinger, Jörg
AU - Arold, Stefan T.
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): URF/1/1976-06
Acknowledgements: The research reported in this publication was supported by King Abdullah University of Science and Technology (KAUST) through the baseline fund and the Award No URF/1/1976-06 from the Office of Sponsored Research (OSR).
PY - 2018/3/6
Y1 - 2018/3/6
N2 - The fermentation of carbon dioxide (CO2) with hydrogen (H2) uses available low-cost gases to synthesis acetic acid. Here, we present a two-stage biological process that allows the gas to liquid transfer (Bio-GTL) of CO2 into the biopolymer polyhydroxybutyrate (PHB). Using the same medium in both stages, first, acetic acid is produced (3.2 g L−1) by Acetobacterium woodii from 5.2 L gas-mixture of CO2:H2 (15:85 v/v) under elevated pressure (≥2.0 bar) to increase H2-solubility in water. Second, acetic acid is converted to PHB (3 g L−1 acetate into 0.5 g L−1 PHB) by Ralstonia eutropha H16. The efficiencies and space-time yields were evaluated, and our data show the conversion of CO2 into PHB with a 33.3% microbial cell content (percentage of the ratio of PHB concentration to cell concentration) after 217 h. Collectively, our results provide a resourceful platform for future optimization and commercialization of a Bio-GTL for PHB production.
AB - The fermentation of carbon dioxide (CO2) with hydrogen (H2) uses available low-cost gases to synthesis acetic acid. Here, we present a two-stage biological process that allows the gas to liquid transfer (Bio-GTL) of CO2 into the biopolymer polyhydroxybutyrate (PHB). Using the same medium in both stages, first, acetic acid is produced (3.2 g L−1) by Acetobacterium woodii from 5.2 L gas-mixture of CO2:H2 (15:85 v/v) under elevated pressure (≥2.0 bar) to increase H2-solubility in water. Second, acetic acid is converted to PHB (3 g L−1 acetate into 0.5 g L−1 PHB) by Ralstonia eutropha H16. The efficiencies and space-time yields were evaluated, and our data show the conversion of CO2 into PHB with a 33.3% microbial cell content (percentage of the ratio of PHB concentration to cell concentration) after 217 h. Collectively, our results provide a resourceful platform for future optimization and commercialization of a Bio-GTL for PHB production.
UR - http://hdl.handle.net/10754/627316
UR - http://www.sciencedirect.com/science/article/pii/S2589014X18300148
U2 - 10.1016/j.biteb.2018.02.007
DO - 10.1016/j.biteb.2018.02.007
M3 - Article
SN - 2589-014X
VL - 1
SP - 61
EP - 68
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
ER -