Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

Jessica L. Cohen; Stephanie Schubert; Peter R. Wich; Lina Cui; Joel A. Cohen; Justin L. Mynar; Jean M.J. Fréchet

doi:10.1021/bc100542r

Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

Jessica L. Cohen, Stephanie Schubert, Peter R. Wich, Lina Cui, Joel A. Cohen, Justin L. Mynar, Jean M.J. Fréchet

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

134 Scopus citations

Abstract

We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

Original language	English (US)
Pages (from-to)	1056-1065
Number of pages	10
Journal	Bioconjugate Chemistry
Volume	22
Issue number	6
DOIs	https://doi.org/10.1021/bc100542r
State	Published - Jun 15 2011

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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abstract = "We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.",

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AU - Cohen, Jessica L.

AU - Schubert, Stephanie

AU - Wich, Peter R.

AU - Cui, Lina

AU - Cohen, Joel A.

AU - Mynar, Justin L.

AU - Fréchet, Jean M.J.

PY - 2011/6/15

Y1 - 2011/6/15

N2 - We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

AB - We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

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Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

Abstract

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