Acquisition and Analysis of Data from High Concentration Solutions

Tabot M.D. Besong, Arthur J. Rowe

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


The problems associated with ultracentrifugal analysis of macromolecular solutions at high (>10 mg/ml) are reviewed. Especially for the case of solutes which are non-monodisperse, meaningful results are not readily achievable using sedimentation velocity approaches. It is shown however by both simulation and analysis of practical data that using a modified form of an algorithm (INVEQ) published in other contexts, sedimentation equilibrium (SE) profiles can be analysed successfully, enabling topics such as oligomer presence or formation to be defined.To achieve this, it is necessary to employ an approach in which the solution density, which in an SE profile is radius-dependent, is taken into consideration. Simulation suggests that any reasonable level of solute concentration can be analysed.
Original languageEnglish (US)
Title of host publicationAnalytical Ultracentrifugation
PublisherSpringer Nature
Number of pages22
ISBN (Print)9784431559832
StatePublished - May 14 2016


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