Actions of VIP, hGRF, PHI and secretin: Comparative studies in cerebral cortex and adenohypophysis

Pierre J. Magistretti; Philippe Schönenberg; Philippe Kehrer; Jean Luc Martin; Rolf C. Gaillard

doi:10.1016/0196-9781(86)90182-8

Actions of VIP, hGRF, PHI and secretin: Comparative studies in cerebral cortex and adenohypophysis

Pierre J. Magistretti^*, Philippe Schönenberg, Philippe Kehrer, Jean Luc Martin, Rolf C. Gaillard

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We have examined the effects of hGRF on cyclic AMP and glycogen levels in mouse cerebral cortical slices. hGRF-44-NH₂ and hGRF-28-OH did not stimulate cyclic AMP formation nor glycogenolysis and did not antagonize the stimulatory effects of VIP on cyclic AMP formation and glycogenolysis. These observations indicate that despite the structural homologies with VIP, hGRF does not interact with VIP receptors coupled to adenylate cyclase in mouse cerebral cortex. This is in contrast with observations in other tissues and species, such as rat and human intestinal epithelial membranes [18] and rat pancreas [34]. We have also compared the effects of hGRF, VIP, PHI and secretin on Growth Hormone (GH) release and cyclic AMP levels in anterior pituitary cells in vitro. VIP and PHI, but not secretin, promote at a high concentration (10^-6 M) a small but significant release of GH. This GH release is accompanied by increases in cyclic AMP levels. The concentration of VIP and PHI required to elicit these effects is high and suggests that VIP and PHI act as low affinity pharmacological analogs of hGRF on hGRF pituitary receptors.

Original language	English (US)
Pages (from-to)	175-180
Number of pages	6
Journal	Peptides
Volume	7
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/0196-9781(86)90182-8
State	Published - 1986
Externally published	Yes

Keywords

Binding
Cerebral cortex
Cultures
Cyclic AMP
GRF
Glycogenolysis
Growth Hormone
Receptors
VIP

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(86)90182-8

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title = "Actions of VIP, hGRF, PHI and secretin: Comparative studies in cerebral cortex and adenohypophysis",

abstract = "We have examined the effects of hGRF on cyclic AMP and glycogen levels in mouse cerebral cortical slices. hGRF-44-NH2 and hGRF-28-OH did not stimulate cyclic AMP formation nor glycogenolysis and did not antagonize the stimulatory effects of VIP on cyclic AMP formation and glycogenolysis. These observations indicate that despite the structural homologies with VIP, hGRF does not interact with VIP receptors coupled to adenylate cyclase in mouse cerebral cortex. This is in contrast with observations in other tissues and species, such as rat and human intestinal epithelial membranes [18] and rat pancreas [34]. We have also compared the effects of hGRF, VIP, PHI and secretin on Growth Hormone (GH) release and cyclic AMP levels in anterior pituitary cells in vitro. VIP and PHI, but not secretin, promote at a high concentration (10-6 M) a small but significant release of GH. This GH release is accompanied by increases in cyclic AMP levels. The concentration of VIP and PHI required to elicit these effects is high and suggests that VIP and PHI act as low affinity pharmacological analogs of hGRF on hGRF pituitary receptors.",

keywords = "Binding, Cerebral cortex, Cultures, Cyclic AMP, GRF, Glycogenolysis, Growth Hormone, Receptors, VIP",

author = "Magistretti, {Pierre J.} and Philippe Sch{\"o}nenberg and Philippe Kehrer and Martin, {Jean Luc} and Gaillard, {Rolf C.}",

year = "1986",

doi = "10.1016/0196-9781(86)90182-8",

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T2 - Comparative studies in cerebral cortex and adenohypophysis

AU - Magistretti, Pierre J.

AU - Schönenberg, Philippe

AU - Kehrer, Philippe

AU - Martin, Jean Luc

AU - Gaillard, Rolf C.

PY - 1986

Y1 - 1986

N2 - We have examined the effects of hGRF on cyclic AMP and glycogen levels in mouse cerebral cortical slices. hGRF-44-NH2 and hGRF-28-OH did not stimulate cyclic AMP formation nor glycogenolysis and did not antagonize the stimulatory effects of VIP on cyclic AMP formation and glycogenolysis. These observations indicate that despite the structural homologies with VIP, hGRF does not interact with VIP receptors coupled to adenylate cyclase in mouse cerebral cortex. This is in contrast with observations in other tissues and species, such as rat and human intestinal epithelial membranes [18] and rat pancreas [34]. We have also compared the effects of hGRF, VIP, PHI and secretin on Growth Hormone (GH) release and cyclic AMP levels in anterior pituitary cells in vitro. VIP and PHI, but not secretin, promote at a high concentration (10-6 M) a small but significant release of GH. This GH release is accompanied by increases in cyclic AMP levels. The concentration of VIP and PHI required to elicit these effects is high and suggests that VIP and PHI act as low affinity pharmacological analogs of hGRF on hGRF pituitary receptors.

AB - We have examined the effects of hGRF on cyclic AMP and glycogen levels in mouse cerebral cortical slices. hGRF-44-NH2 and hGRF-28-OH did not stimulate cyclic AMP formation nor glycogenolysis and did not antagonize the stimulatory effects of VIP on cyclic AMP formation and glycogenolysis. These observations indicate that despite the structural homologies with VIP, hGRF does not interact with VIP receptors coupled to adenylate cyclase in mouse cerebral cortex. This is in contrast with observations in other tissues and species, such as rat and human intestinal epithelial membranes [18] and rat pancreas [34]. We have also compared the effects of hGRF, VIP, PHI and secretin on Growth Hormone (GH) release and cyclic AMP levels in anterior pituitary cells in vitro. VIP and PHI, but not secretin, promote at a high concentration (10-6 M) a small but significant release of GH. This GH release is accompanied by increases in cyclic AMP levels. The concentration of VIP and PHI required to elicit these effects is high and suggests that VIP and PHI act as low affinity pharmacological analogs of hGRF on hGRF pituitary receptors.

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KW - Growth Hormone

KW - Receptors

KW - VIP

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ER -

Actions of VIP, hGRF, PHI and secretin: Comparative studies in cerebral cortex and adenohypophysis

Abstract

Keywords

ASJC Scopus subject areas

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