TY - JOUR
T1 - Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62
AU - Yu, Guotai
AU - Matny, Oadi
AU - Champouret, Nicolas
AU - Steuernagel, Burkhard
AU - Moscou, Matthew J.
AU - Hernández-Pinzón, Inmaculada
AU - Green, Phon
AU - Hayta, Sadiye
AU - Smedley, Mark
AU - Harwood, Wendy
AU - Kangara, Ngonidzashe
AU - Yue, Yajuan
AU - Gardener, Catherine
AU - Banfield, Mark J.
AU - Olivera, Pablo D.
AU - Welchin, Cole
AU - Simmons, Jamie
AU - Millet, Eitan
AU - Minz-Dub, Anna
AU - Ronen, Moshe
AU - Avni, Raz
AU - Sharon, Amir
AU - Patpour, Mehran
AU - Justesen, Annemarie F.
AU - Jayakodi, Murukarthick
AU - Himmelbach, Axel
AU - Stein, Nils
AU - Wu, Shuangye
AU - Poland, Jesse
AU - Ens, Jennifer
AU - Pozniak, Curtis
AU - Karafiátová, Miroslava
AU - Molnár, István
AU - Doležel, Jaroslav
AU - Ward, Eric R.
AU - Reuber, T. Lynne
AU - Jones, Jonathan D.G.
AU - Mascher, Martin
AU - Steffenson, Brian J.
AU - Wulff, Brande B.H.
N1 - Funding Information:
We are grateful to the Harold and Adele Lieberman Germplasm Bank (Tel Aviv University), Hazera Seeds Ltd and Limagrain for making germplasm available. We thank Ryan Johnson for phenotyping some of the Sr62 introgression line mutants, Yue Jin for supplying several of the stem rust cultures, JIC Horticultural Services for plant husbandry, Matthew Heaton for assistance with figure design, Manuela Knauft and Ines Walde for technical assistance on Hi-C library preparation and sequencing, Anne Fiebig for sequence data submission, Jan Vrána, Zdeňka Dubská and Romana Šperková for assistance with chromosome sorting and DNA amplification, Bob McIntosh for review of the draft manuscript, and Willem Boshoff for helpful discussion. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by grants from the 2Blades Foundation, USA, to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. (BBS/E/J/000PR9780); the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; Human Frontier Science Program long-term fellowship (LT000218/2011-L) to M.J.M.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the 2Blades Foundation; and the Gatsby Charitable Foundation to J.D.G.J.
Funding Information:
We are grateful to the Harold and Adele Lieberman Germplasm Bank (Tel Aviv University), Hazera Seeds Ltd and Limagrain for making germplasm available. We thank Ryan Johnson for phenotyping some of the Sr62 introgression line mutants, Yue Jin for supplying several of the stem rust cultures,?JIC Horticultural Services for plant husbandry, Matthew Heaton for assistance with figure design, Manuela Knauft and Ines Walde for technical assistance on Hi-C library preparation and sequencing, Anne Fiebig for sequence data submission, Jan Vr?na, Zde?ka Dubsk? and Romana ?perkov? for assistance with chromosome sorting and DNA amplification, Bob McIntosh for review of the draft manuscript, and Willem Boshoff for helpful discussion. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by grants from the 2Blades Foundation, USA, to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. (BBS/E/J/000PR9780); the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; Human Frontier Science Program long-term fellowship (LT000218/2011-L) to M.J.M.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the 2Blades Foundation; and the Gatsby Charitable Foundation to J.D.G.J.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.
AB - The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.
UR - http://www.scopus.com/inward/record.url?scp=85127282541&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-29132-8
DO - 10.1038/s41467-022-29132-8
M3 - Article
C2 - 35338132
AN - SCOPUS:85127282541
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1607
ER -