TY - CHAP
T1 - Affinity Purification Protocol Starting with a Small Molecule as Bait
AU - Figueroa, Nicolás E.
AU - Hernandez-Sanchez, Itzell E.
AU - Maruri-Lopez, Israel
AU - Chodasiewicz, Monika
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Protein–metabolite interactions (PMIs) are fundamental for several biological processes. Even though PMI studies have increased in recent years, our knowledge is still limited. The screening of PMIs using small molecules as bait will broaden our ability to uncover novel PMIs, setting the basis for establishing their biological relevance. Here, we describe a protocol that allows the identification of multiple protein partners for one ligand. This protocol describes a straightforward methodology that can be adapted to a wide variety of organisms.
AB - Protein–metabolite interactions (PMIs) are fundamental for several biological processes. Even though PMI studies have increased in recent years, our knowledge is still limited. The screening of PMIs using small molecules as bait will broaden our ability to uncover novel PMIs, setting the basis for establishing their biological relevance. Here, we describe a protocol that allows the identification of multiple protein partners for one ligand. This protocol describes a straightforward methodology that can be adapted to a wide variety of organisms.
KW - Affinity purification
KW - Ligand
KW - Mass spectrometry
KW - Metabolites
KW - Protein–metabolite interactions
KW - Protein–protein interactions
UR - http://www.scopus.com/inward/record.url?scp=85139531997&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2624-5_2
DO - 10.1007/978-1-0716-2624-5_2
M3 - Chapter
C2 - 36178617
AN - SCOPUS:85139531997
T3 - Methods in Molecular Biology
SP - 11
EP - 19
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -