Affinity Purification Protocol Starting with a Small Molecule as Bait

Nicolás E. Figueroa, Itzell E. Hernandez-Sanchez, Israel Maruri-Lopez, Monika Chodasiewicz*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Protein–metabolite interactions (PMIs) are fundamental for several biological processes. Even though PMI studies have increased in recent years, our knowledge is still limited. The screening of PMIs using small molecules as bait will broaden our ability to uncover novel PMIs, setting the basis for establishing their biological relevance. Here, we describe a protocol that allows the identification of multiple protein partners for one ligand. This protocol describes a straightforward methodology that can be adapted to a wide variety of organisms.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Affinity purification
  • Ligand
  • Mass spectrometry
  • Metabolites
  • Protein–metabolite interactions
  • Protein–protein interactions

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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