TY - JOUR
T1 - An integrated physical, genetic and cytogenetic map of Brachypodium distachyon, a model system for grass research
AU - Febrer, Melanie
AU - Goicoechea, Jose Luis
AU - Wright, Jonathan
AU - McKenzie, Neil
AU - Song, Xiang
AU - Lin, Jinke
AU - Collura, Kristi
AU - Wissotski, Marina
AU - Yu, Yeisoo
AU - Ammiraju, Jetty S.S.
AU - Wolny, Elzbieta
AU - Idziak, Dominika
AU - Betekhtin, Alexander
AU - Kudrna, Dave
AU - Hasterok, Robert
AU - Wing, Rod A.
AU - Bevan, Michael W.
N1 - Generated from Scopus record by KAUST IRTS on 2019-11-20
PY - 2010/11/17
Y1 - 2010/11/17
N2 - The pooid subfamily of grasses includes some of the most important crop, forage and turf species, such as wheat, barley and Lolium. Developing genomic resources, such as whole-genome physical maps, for analysing the large and complex genomes of these crops and for facilitating biological research in grasses is an important goal in plant biology. We describe a bacterial artificial chromosome (BAC)-based physical map of the wild pooid grass Brachypodium distachyon and integrate this with whole genome shotgun sequence (WGS) assemblies using BAC end sequences (BES). The resulting physical map contains 26 contigs spanning the 272 Mb genome. BES from the physical map were also used to integrate a genetic map. This provides an independent vaildation and confirmation of the published WGS assembly. Mapped BACs were used in Fluorescence In Situ Hybridisation (FISH) experiments to align the integrated physical map and sequence assemblies to chromosomes with high resolution. The physical, genetic and cytogenetic maps, integrated with whole genome shotgun sequence assemblies, enhance the accuracy and durability of this important genome sequence and will directly facilitate gene isolation. © 2010 Febrer et al.
AB - The pooid subfamily of grasses includes some of the most important crop, forage and turf species, such as wheat, barley and Lolium. Developing genomic resources, such as whole-genome physical maps, for analysing the large and complex genomes of these crops and for facilitating biological research in grasses is an important goal in plant biology. We describe a bacterial artificial chromosome (BAC)-based physical map of the wild pooid grass Brachypodium distachyon and integrate this with whole genome shotgun sequence (WGS) assemblies using BAC end sequences (BES). The resulting physical map contains 26 contigs spanning the 272 Mb genome. BES from the physical map were also used to integrate a genetic map. This provides an independent vaildation and confirmation of the published WGS assembly. Mapped BACs were used in Fluorescence In Situ Hybridisation (FISH) experiments to align the integrated physical map and sequence assemblies to chromosomes with high resolution. The physical, genetic and cytogenetic maps, integrated with whole genome shotgun sequence assemblies, enhance the accuracy and durability of this important genome sequence and will directly facilitate gene isolation. © 2010 Febrer et al.
UR - https://dx.plos.org/10.1371/journal.pone.0013461
UR - http://www.scopus.com/inward/record.url?scp=78149437473&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0013461
DO - 10.1371/journal.pone.0013461
M3 - Article
SN - 1932-6203
VL - 5
JO - PLoS ONE
JF - PLoS ONE
IS - 10
ER -