TY - JOUR
T1 - An SGS3-like protein functions in RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsis
AU - Zheng, Zhimin
AU - Xing, Yu
AU - He, Xinjian
AU - Li, Wenbo
AU - Hu, Yuanlei
AU - Yadav, Sudesh Kumar
AU - Oh, Jeeeun
AU - Zhu, Jian-Kang
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by National Institutes of Health grant R01GM070795 to J-KZ and a BOYSCAST Fellowship, Department of Science and Technology, Government of India to SKY.
PY - 2010/1/6
Y1 - 2010/1/6
N2 - RNA-directed DNA methylation (RdDM) is an important epigenetic mechanism for silencing transgenes and endogenous repetitive sequences such as transposons. The RD29A promoter-driven LUCIFERASE transgene and its corresponding endogenous RD29A gene are hypermethylated and silenced in the Arabidopsis DNA demethylase mutant ros1. By screening for second-site suppressors of ros1, we identified the RDM12 locus. The rdm12 mutation releases the silencing of the RD29A-LUC transgene and the endogenous RD29A gene by reducing the promoter DNA methylation. The rdm12 mutation also reduces DNA methylation at endogenous RdDM target loci, including transposons and other repetitive sequences. In addition, the rdm12 mutation affects the levels of small interfering RNAs (siRNAs) from some of the RdDM target loci. RDM12 encodes a protein with XS and coiled-coil domains, and is similar to SGS3, which is a partner protein of RDR6 and can bind to double-stranded RNAs with a 5′ overhang, and is required for several post-transcriptional gene silencing pathways. Our results show that RDM12 is a component of the RdDM pathway, and suggest that RdDM may involve double-stranded RNAs with a 5′ overhang and the partnering between RDM12 and RDR2. © 2010 Blackwell Publishing Ltd.
AB - RNA-directed DNA methylation (RdDM) is an important epigenetic mechanism for silencing transgenes and endogenous repetitive sequences such as transposons. The RD29A promoter-driven LUCIFERASE transgene and its corresponding endogenous RD29A gene are hypermethylated and silenced in the Arabidopsis DNA demethylase mutant ros1. By screening for second-site suppressors of ros1, we identified the RDM12 locus. The rdm12 mutation releases the silencing of the RD29A-LUC transgene and the endogenous RD29A gene by reducing the promoter DNA methylation. The rdm12 mutation also reduces DNA methylation at endogenous RdDM target loci, including transposons and other repetitive sequences. In addition, the rdm12 mutation affects the levels of small interfering RNAs (siRNAs) from some of the RdDM target loci. RDM12 encodes a protein with XS and coiled-coil domains, and is similar to SGS3, which is a partner protein of RDR6 and can bind to double-stranded RNAs with a 5′ overhang, and is required for several post-transcriptional gene silencing pathways. Our results show that RDM12 is a component of the RdDM pathway, and suggest that RdDM may involve double-stranded RNAs with a 5′ overhang and the partnering between RDM12 and RDR2. © 2010 Blackwell Publishing Ltd.
UR - http://hdl.handle.net/10754/561468
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858770
UR - http://www.scopus.com/inward/record.url?scp=77951824122&partnerID=8YFLogxK
U2 - 10.1111/j.1365-313X.2010.04130.x
DO - 10.1111/j.1365-313X.2010.04130.x
M3 - Article
C2 - 20059743
SN - 0960-7412
VL - 62
SP - 92
EP - 99
JO - The Plant Journal
JF - The Plant Journal
IS - 1
ER -