TY - CHAP
T1 - Analyzing Lysosome-Related Organelles by Electron Microscopy
AU - Hurbain, Ilse
AU - Romao, Maryse
AU - Bergam, Ptissam
AU - Heiligenstein, Xavier
AU - Raposo, Graça
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.
PY - 2017/4/30
Y1 - 2017/4/30
N2 - Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.
AB - Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.
UR - http://hdl.handle.net/10754/623792
UR - http://link.springer.com/protocol/10.1007%2F978-1-4939-6934-0_4
UR - http://www.scopus.com/inward/record.url?scp=85018241964&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6934-0_4
DO - 10.1007/978-1-4939-6934-0_4
M3 - Chapter
SN - 9781493969326
SP - 43
EP - 71
BT - Methods in Molecular Biology
PB - Springer Nature
ER -