TY - JOUR
T1 - Aquasomes - A nanoparticulate approach for the delivery of antigen
AU - Goyal, Amit K.
AU - Khatri, Kapil
AU - Mishra, Neeraj
AU - Mehta, Abhinav
AU - Vaidya, Bhuvaneshwar
AU - Tiwari, Shailja
AU - Vyas, Suresh Prasad
N1 - Generated from Scopus record by KAUST IRTS on 2023-10-12
PY - 2008/12/1
Y1 - 2008/12/1
N2 - The development of compound that enhances immune responses to recombinant or synthetic epitopes is of considerable importance in vaccine research. Of the many different types of immunopotentiating compounds that have been researched, aquasomes are of considerable promise, because of their potency and adjuvanticity. Aquasomes were prepared by self-assembling of hydroxyapatite by co-precipitation method and thereafter preliminary coated with polyhydroxyl oligomers (cellobiose and trehalose) and subsequently adsorbed with bovine serum albumin (BSA) as a model antigen. The prepared systems were characterized for size, shape, antigen-loading efficiency, in vitro antigen stability, and in vivo performance. BSA-immobilized aquasomes were around 200 nm in diameter and spherical in shape and had approximately 20-30% BSA-loading efficiency. The immunological activity of the formulated aquasomes was compared with plain BSA and better results were observed. Studies also indicated that aquasome formulations could elicit combined T-helper 1 (Th1) and Th2 immune response. Copyright © Informa UK, Ltd.
AB - The development of compound that enhances immune responses to recombinant or synthetic epitopes is of considerable importance in vaccine research. Of the many different types of immunopotentiating compounds that have been researched, aquasomes are of considerable promise, because of their potency and adjuvanticity. Aquasomes were prepared by self-assembling of hydroxyapatite by co-precipitation method and thereafter preliminary coated with polyhydroxyl oligomers (cellobiose and trehalose) and subsequently adsorbed with bovine serum albumin (BSA) as a model antigen. The prepared systems were characterized for size, shape, antigen-loading efficiency, in vitro antigen stability, and in vivo performance. BSA-immobilized aquasomes were around 200 nm in diameter and spherical in shape and had approximately 20-30% BSA-loading efficiency. The immunological activity of the formulated aquasomes was compared with plain BSA and better results were observed. Studies also indicated that aquasome formulations could elicit combined T-helper 1 (Th1) and Th2 immune response. Copyright © Informa UK, Ltd.
UR - http://www.tandfonline.com/doi/full/10.1080/03639040802071661
UR - http://www.scopus.com/inward/record.url?scp=57349158687&partnerID=8YFLogxK
U2 - 10.1080/03639040802071661
DO - 10.1080/03639040802071661
M3 - Article
SN - 0363-9045
VL - 34
SP - 1297
EP - 1305
JO - Drug Development and Industrial Pharmacy
JF - Drug Development and Industrial Pharmacy
IS - 12
ER -
