Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans

Karen L. Artiles, Andrew Z. Fire*, Christian Frøkjær-Jensen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.

Original languageEnglish (US)
Pages (from-to)827-839.e9
JournalDevelopmental Cell
Issue number6
StatePublished - Mar 25 2019


  • C. elegans
  • genetic engineering
  • inheritance
  • mitosis
  • mosaic
  • non-Mendelian
  • synthetic biology

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Molecular Biology
  • Cell Biology
  • Developmental Biology


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