@article{f1ed21ef0fd54339804d92d49e8c6959,
title = "Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans",
abstract = "The recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.",
keywords = "C. elegans, genetic engineering, inheritance, mitosis, mosaic, non-Mendelian, synthetic biology",
author = "Artiles, {Karen L.} and Fire, {Andrew Z.} and Christian Fr{\o}kj{\ae}r-Jensen",
note = "Funding Information: We thank Henrik Bringmann for communications on the nature of non-canonical inheritance; Elif Sarinay Cenik and Sedona Murphy for observations on the gpr-1 (OE) strains and their genetic interactions; Josh Arribere for the gfp -tagged unc-54 allele; Lamia Wahba for pointing out biological aspects of non-canonical inheritance; Loren Hansen for guidance in SNP calling; Tim Schedl, Michael Ailion, Maria Sallee, and Anne Villeneuve for helpful discussions; Tomoko Tabuchi and Susan Strome for communicating results prior to publication; and Elif Sarinay Cenik, Nimit Jain, Massa Shoura, and Ryan Bell for their critical reading of the manuscript. This work was supported by grant NIGMS - R01-GM37706 / GM130366 (to A.Z.F.). Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs ( P40 OD010440 ). Funding Information: We thank Henrik Bringmann for communications on the nature of non-canonical inheritance; Elif Sarinay Cenik and Sedona Murphy for observations on the gpr-1(OE) strains and their genetic interactions; Josh Arribere for the gfp-tagged unc-54 allele; Lamia Wahba for pointing out biological aspects of non-canonical inheritance; Loren Hansen for guidance in SNP calling; Tim Schedl, Michael Ailion, Maria Sallee, and Anne Villeneuve for helpful discussions; Tomoko Tabuchi and Susan Strome for communicating results prior to publication; and Elif Sarinay Cenik, Nimit Jain, Massa Shoura, and Ryan Bell for their critical reading of the manuscript. This work was supported by grant NIGMS-R01-GM37706/GM130366 (to A.Z.F.). Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). K.L.A. A.Z.F. and C.F.-J. conceived the study, shepherded experiments, and wrote the paper. C.F.-J. generated the GPR-1OE strains. K.L.A. generated toolkit strains and performed genetic experiments, imaging, and whole-genome sequencing. A.Z.F. analyzed the maternal and paternal contribution of mitochondrial DNA. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = mar,
day = "25",
doi = "10.1016/j.devcel.2019.01.020",
language = "English (US)",
volume = "48",
pages = "827--839.e9",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "6",
}