Abstract
Bacterial community structure and diversity of Tunisian agricultural soil treated with different amounts of municipal solid waste compost (MSWC) and other fertilizers were studied using DGGE and ARISA fingerprinting methods. Sequence analysis of dominant DGGE bands revealed the presence of three major clusters, Cytophaga/Flexibacter/Bacteroides (CFB) group, Proteobacteria and Acidobacteria group. Using ARISA profiles, dominant populations were assigned to low and high GC Gram positive bacteria, Cyanobacteria, Spirochetes and Cytophagales. The two methods revealed the absence of significant bacterial community shifts related to the different MSWC applications. Moreover, indigenous bacterial population of the used loam-clayey soil was observed to limit proliferation and survival of Proteobacteria, initially dominant in MSWC and farmyard manure. Effectiveness of the two methods for soil bacterial community studying was shown. While DGGE was more accurate for bacterial identification, ARISA was more practical for handling and rapid estimation of dominant bacteria.
Original language | English (US) |
---|---|
Pages (from-to) | 1159-1167 |
Number of pages | 9 |
Journal | World Journal of Microbiology and Biotechnology |
Volume | 24 |
Issue number | 7 |
DOIs | |
State | Published - Jul 2008 |
Externally published | Yes |
Keywords
- ARISA
- Bacterial community structure
- Compost
- DGGE
ASJC Scopus subject areas
- Biotechnology
- Physiology
- Applied Microbiology and Biotechnology