Bacterial Footprints in Elastic Pillared Microstructures

Arturo Susarrey-Arce, Jose Federico Hernandez Sanchez, Marco Marcello, Yuri Diaz-Fernandez, Alina Oknianska, Ioritz Sorzabal-Bellido, Roald Tiggelaar, Detlef Lohse, Han Gardeniers, Jacco Snoeijer, Alvaro Marin, Rasmita Raval

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Soft substrates decorated with micropillar arrays are known to be sensitive to deflection due to capillary action. In this work, we demonstrate that micropillared epoxy surfaces are sensitive to single drops of bacterial suspensions. The micropillars can show significant deformations upon evaporation, just as capillary action does in soft substrates. The phenomenon has been studied with five bacterial strains: S. epidermidis, L. sakei, P. aeruginosa, E. coli, and B. subtilis. The results reveal that only droplets containing motile microbes with flagella stimulate micropillar bending, which leads to significant distortions and pillar aggregations forming dimers, trimers, and higher order clusters. Such deformation is manifested in characteristic patterns that are left on the microarrayed surface following evaporation and can be easily identified even by the naked eye. Our findings could lay the ground for the design and fabrication of mechanically responsive substrates, sensitive to specific types of microorganisms.
Original languageEnglish (US)
Pages (from-to)1294-1300
Number of pages7
JournalACS Applied Bio Materials
Issue number5
StatePublished - Oct 15 2018


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