TY - JOUR
T1 - Binary transition metal oxide modified laser-scribed graphene electrochemical aptasensor for the accurate and sensitive screening of acute myocardial infarction
AU - Rauf, Sakandar
AU - Mani, Veerappan
AU - Ait Lahcen, Abdellatif
AU - Yuvaraja, Saravanan
AU - Beduk, Tutku
AU - Salama, Khaled N.
N1 - KAUST Repository Item: Exported on 2021-05-27
Acknowledged KAUST grant number(s): KAUST Sensor Initiative
Acknowledgements: The authors would like to express their acknowledgments to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative for supporting this work. In addition, we would like to thank Dr. Govindasamy Mani for his valuable suggestions regarding material synthesis.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Laser-scribed graphene (LSG) electrodes have gained significant interest due to the ease in fabrication, surface modification, and potential to develop various types of electrochemical sensors and biosensors. In these studies, a new type of zinc ferrite nanoparticles (ZnFe2O4 NPs) modified LSG electrochemical sensing system comprising LSG-ZnFe2O4 working electrode, LSG reference, and LSG counter electrode on a single polyimide substrate is presented. LSG-ZnFe2O4 electrodes are fabricated by drop-casting a solution of ZnFe2O4 NPs onto the LSG electrode, which gave a 31% enhancement of sensitivity and electrocatalytic activity compared to the bare LSG electrode. LSG-ZnFe2O4 electrochemical aptasensor for acute myocardial infarction (AMI) screening is developed by detecting the cardiac Troponin-I (cTn-I) biomarker. The results show that the developed aptasensor could detect a broad concentration range of cTn-I with a limit of detection of 0.001 ng/mL and a sensitivity of 19.32 (±0.25) µA/(ng/mL). In addition to this, LSG-ZnFe2O4-aptasensor shows higher selectivity towards the detection of cTn-I and negligible cross-reactivity with other interfering biomolecules. Finally, it is demonstrated that LSG-ZnFe2O4-aptasensor can easily detect different concentrations of cTn-I spiked in human serum samples. These results show that the LSG-ZnFe2O4-aptasensor is a promising diagnostic tool to monitor cTn-I and could be a potential candidate to develop point-of-care devices for cTn-I biomarker detection and various other disease biomarkers in the future.
AB - Laser-scribed graphene (LSG) electrodes have gained significant interest due to the ease in fabrication, surface modification, and potential to develop various types of electrochemical sensors and biosensors. In these studies, a new type of zinc ferrite nanoparticles (ZnFe2O4 NPs) modified LSG electrochemical sensing system comprising LSG-ZnFe2O4 working electrode, LSG reference, and LSG counter electrode on a single polyimide substrate is presented. LSG-ZnFe2O4 electrodes are fabricated by drop-casting a solution of ZnFe2O4 NPs onto the LSG electrode, which gave a 31% enhancement of sensitivity and electrocatalytic activity compared to the bare LSG electrode. LSG-ZnFe2O4 electrochemical aptasensor for acute myocardial infarction (AMI) screening is developed by detecting the cardiac Troponin-I (cTn-I) biomarker. The results show that the developed aptasensor could detect a broad concentration range of cTn-I with a limit of detection of 0.001 ng/mL and a sensitivity of 19.32 (±0.25) µA/(ng/mL). In addition to this, LSG-ZnFe2O4-aptasensor shows higher selectivity towards the detection of cTn-I and negligible cross-reactivity with other interfering biomolecules. Finally, it is demonstrated that LSG-ZnFe2O4-aptasensor can easily detect different concentrations of cTn-I spiked in human serum samples. These results show that the LSG-ZnFe2O4-aptasensor is a promising diagnostic tool to monitor cTn-I and could be a potential candidate to develop point-of-care devices for cTn-I biomarker detection and various other disease biomarkers in the future.
UR - http://hdl.handle.net/10754/669272
UR - https://linkinghub.elsevier.com/retrieve/pii/S0013468621007799
UR - http://www.scopus.com/inward/record.url?scp=85105712896&partnerID=8YFLogxK
U2 - 10.1016/j.electacta.2021.138489
DO - 10.1016/j.electacta.2021.138489
M3 - Article
SN - 0013-4686
VL - 386
SP - 138489
JO - Electrochimica Acta
JF - Electrochimica Acta
ER -
