Biochemical aspects of carotene desaturation and cyclization in chromoplast membranes from Narcissus pseudonarcissus

Peter Beyer; Vitus Nievelstein; Salim Albabili; Michael Bonk; Hans Kleinig

doi:10.1351/pac199466051047

Biochemical aspects of carotene desaturation and cyclization in chromoplast membranes from Narcissus pseudonarcissus

Peter Beyer, Vitus Nievelstein, Salim Albabili, Michael Bonk, Hans Kleinig

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Abstract

In Narcissus chromoplast membranes, phytoene is converted into coloured carotenes by two desaturases which differ in stereospecificity and which can be distinguished by herbicidal compounds. The desaturases represent flavoproteins, which gain FAD in an assisted folding pathway after being imported into the organelle. Electrons liberated in the desaturation are transferred to oxygen via an intermediate quinone carrier. The possibility of a co-reduction mechanism is discussed. The redox-state of quinones is regulated by an NADPH: quinone oxidoreductase which is membrane-peripherally localized. The end product of the desaturation, 7,9,7 ‘, 9 ‘-tetra-cis-lycopene (prolycopene), is the preferred substrate for cyclization, which is accompanied by an NADPH-dependent isomerization yielding trans cyclic products.

Original language	English (US)
Pages (from-to)	1047-1056
Number of pages	10
Journal	Pure and Applied Chemistry
Volume	66
Issue number	5
DOIs	https://doi.org/10.1351/pac199466051047
State	Published - Jan 1 1994
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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title = "Biochemical aspects of carotene desaturation and cyclization in chromoplast membranes from Narcissus pseudonarcissus",

abstract = "In Narcissus chromoplast membranes, phytoene is converted into coloured carotenes by two desaturases which differ in stereospecificity and which can be distinguished by herbicidal compounds. The desaturases represent flavoproteins, which gain FAD in an assisted folding pathway after being imported into the organelle. Electrons liberated in the desaturation are transferred to oxygen via an intermediate quinone carrier. The possibility of a co-reduction mechanism is discussed. The redox-state of quinones is regulated by an NADPH: quinone oxidoreductase which is membrane-peripherally localized. The end product of the desaturation, 7,9,7 {\textquoteleft}, 9 {\textquoteleft}-tetra-cis-lycopene (prolycopene), is the preferred substrate for cyclization, which is accompanied by an NADPH-dependent isomerization yielding trans cyclic products.",

author = "Peter Beyer and Vitus Nievelstein and Salim Albabili and Michael Bonk and Hans Kleinig",

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T1 - Biochemical aspects of carotene desaturation and cyclization in chromoplast membranes from Narcissus pseudonarcissus

AU - Beyer, Peter

AU - Nievelstein, Vitus

AU - Albabili, Salim

AU - Bonk, Michael

AU - Kleinig, Hans

PY - 1994/1/1

Y1 - 1994/1/1

N2 - In Narcissus chromoplast membranes, phytoene is converted into coloured carotenes by two desaturases which differ in stereospecificity and which can be distinguished by herbicidal compounds. The desaturases represent flavoproteins, which gain FAD in an assisted folding pathway after being imported into the organelle. Electrons liberated in the desaturation are transferred to oxygen via an intermediate quinone carrier. The possibility of a co-reduction mechanism is discussed. The redox-state of quinones is regulated by an NADPH: quinone oxidoreductase which is membrane-peripherally localized. The end product of the desaturation, 7,9,7 ‘, 9 ‘-tetra-cis-lycopene (prolycopene), is the preferred substrate for cyclization, which is accompanied by an NADPH-dependent isomerization yielding trans cyclic products.

AB - In Narcissus chromoplast membranes, phytoene is converted into coloured carotenes by two desaturases which differ in stereospecificity and which can be distinguished by herbicidal compounds. The desaturases represent flavoproteins, which gain FAD in an assisted folding pathway after being imported into the organelle. Electrons liberated in the desaturation are transferred to oxygen via an intermediate quinone carrier. The possibility of a co-reduction mechanism is discussed. The redox-state of quinones is regulated by an NADPH: quinone oxidoreductase which is membrane-peripherally localized. The end product of the desaturation, 7,9,7 ‘, 9 ‘-tetra-cis-lycopene (prolycopene), is the preferred substrate for cyclization, which is accompanied by an NADPH-dependent isomerization yielding trans cyclic products.

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Biochemical aspects of carotene desaturation and cyclization in chromoplast membranes from Narcissus pseudonarcissus

Abstract

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