TY - JOUR
T1 - Biofunctionalized Two-Dimensional Ti3C2 MXenes for Ultrasensitive Detection of Cancer Biomarker
AU - Kumar, Saurabh
AU - Lei, Yongjiu
AU - Alshareef, Niman H.
AU - Quevedo-Lopez, M.A.
AU - Salama, Khaled N.
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank Prof. Sahika Inal for providing the serum sample. Research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST).
PY - 2018/8/31
Y1 - 2018/8/31
N2 - In this work, ultrathin Ti3C2-MXene nanosheets were synthesized by minimally intensive layer delamination methods, and uniformly functionalized with aminosilane (f-Ti3C2-MXene) to provide a covalent binding for the immobilized bio-receptor (anti-CEA) for label free, ultrasensitive detection of cancer biomarker (carcinoembryonic antigen, CEA). The effect of different redox probes on the electrochemical behavior of f-Ti3C2-MXene was investigated and found that hexaammineruthenium ([Ru(NH3)6]3+) is the preferable redox probe for biosensing. The fabricated biofunctionalized Ti3C2-MXene exhibits a linear detection range of 0.0001–2000 ngmL−1 with sensitivity of 37.9 µAng−1mLcm−2 per decade. The wider linear detection range of our f-Ti3C2-MXene is not only higher than previously reported pristine 2D nanomaterials, but is even comparable to other hybrid 2D nanomaterials. We believe that this work opens a new window for development of MXene-based highly sensitive DNA, aptamer, enzyme, antibody, and cell based biosensors, and could be further used in drug delivery application.
AB - In this work, ultrathin Ti3C2-MXene nanosheets were synthesized by minimally intensive layer delamination methods, and uniformly functionalized with aminosilane (f-Ti3C2-MXene) to provide a covalent binding for the immobilized bio-receptor (anti-CEA) for label free, ultrasensitive detection of cancer biomarker (carcinoembryonic antigen, CEA). The effect of different redox probes on the electrochemical behavior of f-Ti3C2-MXene was investigated and found that hexaammineruthenium ([Ru(NH3)6]3+) is the preferable redox probe for biosensing. The fabricated biofunctionalized Ti3C2-MXene exhibits a linear detection range of 0.0001–2000 ngmL−1 with sensitivity of 37.9 µAng−1mLcm−2 per decade. The wider linear detection range of our f-Ti3C2-MXene is not only higher than previously reported pristine 2D nanomaterials, but is even comparable to other hybrid 2D nanomaterials. We believe that this work opens a new window for development of MXene-based highly sensitive DNA, aptamer, enzyme, antibody, and cell based biosensors, and could be further used in drug delivery application.
UR - http://hdl.handle.net/10754/628698
UR - https://www.sciencedirect.com/science/article/pii/S0956566318306857
UR - http://www.scopus.com/inward/record.url?scp=85053392238&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2018.08.076
DO - 10.1016/j.bios.2018.08.076
M3 - Article
C2 - 30219724
SN - 0956-5663
VL - 121
SP - 243
EP - 249
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -
