Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice

Shinsaku Ito; Justine Braguy; Jian You Wang; Akiyoshi Yoda; Valentina Fiorilli; Ikuo Takahashi; Muhammad Jamil; Abrar Felemban; Sho Miyazaki; Teresa Mazzarella; Guan Ting Erica Chen; Akihisa Shinozawa; Aparna Balakrishna; Lamis Berqdar; Chakravarty Rajan; Shawkat Ali; Imran Haider; Yasuyuki Sasaki; Shunsuke Yajima; Kohki Akiyama; Luisa Lanfranco; Matias D. Zurbriggen; Takahito Nomura; Tadao Asami; Salim Al-Babili

doi:10.1126/sciadv.add1278

Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice

Shinsaku Ito, Justine Braguy, Jian You Wang, Akiyoshi Yoda, Valentina Fiorilli, Ikuo Takahashi, Muhammad Jamil, Abrar Felemban, Sho Miyazaki, Teresa Mazzarella, Guan Ting Erica Chen, Akihisa Shinozawa, Aparna Balakrishna, Lamis Berqdar, Chakravarty Rajan, Shawkat Ali, Imran Haider, Yasuyuki Sasaki, Shunsuke Yajima, Kohki AkiyamaLuisa Lanfranco, Matias D. Zurbriggen, Takahito Nomura^*, Tadao Asami^*, Salim Al-Babili^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Strigolactones (SLs) are a plant hormone inhibiting shoot branching/tillering and a rhizospheric, chemical signal that triggers seed germination of the noxious root parasitic plant Striga and mediates symbiosis with beneficial arbuscular mycorrhizal fungi. Identifying specific roles of canonical and noncanonical SLs, the two SL subfamilies, is important for developing Striga-resistant cereals and for engineering plant architecture. Here, we report that rice mutants lacking canonical SLs do not show the shoot phenotypes known for SL-deficient plants, exhibiting only a delay in establishing arbuscular mycorrhizal symbiosis, but release exudates with a significantly decreased Striga seed-germinating activity. Blocking the biosynthesis of canonical SLs by TIS108, a specific enzyme inhibitor, significantly lowered Striga infestation without affecting rice growth. These results indicate that canonical SLs are not the determinant of shoot architecture and pave the way for increasing crop resistance by gene editing or chemical treatment.

Original language	English (US)
Article number	add1278
Journal	SCIENCE ADVANCES
Volume	8
Issue number	44
DOIs	https://doi.org/10.1126/sciadv.add1278
State	Published - Nov 2022

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Ito, S., Braguy, J., Wang, J. Y., Yoda, A., Fiorilli, V., Takahashi, I., Jamil, M., Felemban, A., Miyazaki, S., Mazzarella, T., Chen, G. T. E., Shinozawa, A., Balakrishna, A., Berqdar, L., Rajan, C., Ali, S., Haider, I., Sasaki, Y., Yajima, S., ... Al-Babili, S. (2022). Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice. SCIENCE ADVANCES, 8(44), Article add1278. https://doi.org/10.1126/sciadv.add1278

@article{bd99ec3973a4438e926741ef066e69d3,

title = "Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice",

abstract = "Strigolactones (SLs) are a plant hormone inhibiting shoot branching/tillering and a rhizospheric, chemical signal that triggers seed germination of the noxious root parasitic plant Striga and mediates symbiosis with beneficial arbuscular mycorrhizal fungi. Identifying specific roles of canonical and noncanonical SLs, the two SL subfamilies, is important for developing Striga-resistant cereals and for engineering plant architecture. Here, we report that rice mutants lacking canonical SLs do not show the shoot phenotypes known for SL-deficient plants, exhibiting only a delay in establishing arbuscular mycorrhizal symbiosis, but release exudates with a significantly decreased Striga seed-germinating activity. Blocking the biosynthesis of canonical SLs by TIS108, a specific enzyme inhibitor, significantly lowered Striga infestation without affecting rice growth. These results indicate that canonical SLs are not the determinant of shoot architecture and pave the way for increasing crop resistance by gene editing or chemical treatment.",

author = "Shinsaku Ito and Justine Braguy and Wang, {Jian You} and Akiyoshi Yoda and Valentina Fiorilli and Ikuo Takahashi and Muhammad Jamil and Abrar Felemban and Sho Miyazaki and Teresa Mazzarella and Chen, {Guan Ting Erica} and Akihisa Shinozawa and Aparna Balakrishna and Lamis Berqdar and Chakravarty Rajan and Shawkat Ali and Imran Haider and Yasuyuki Sasaki and Shunsuke Yajima and Kohki Akiyama and Luisa Lanfranco and Zurbriggen, {Matias D.} and Takahito Nomura and Tadao Asami and Salim Al-Babili",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors.",

year = "2022",

month = nov,

doi = "10.1126/sciadv.add1278",

language = "English (US)",

volume = "8",

journal = "SCIENCE ADVANCES",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science",

number = "44",

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Ito, S, Braguy, J, Wang, JY, Yoda, A, Fiorilli, V, Takahashi, I, Jamil, M, Felemban, A, Miyazaki, S, Mazzarella, T, Chen, GTE, Shinozawa, A, Balakrishna, A, Berqdar, L, Rajan, C, Ali, S, Haider, I, Sasaki, Y, Yajima, S, Akiyama, K, Lanfranco, L, Zurbriggen, MD, Nomura, T, Asami, T & Al-Babili, S 2022, 'Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice', SCIENCE ADVANCES, vol. 8, no. 44, add1278. https://doi.org/10.1126/sciadv.add1278

TY - JOUR

T1 - Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice

AU - Ito, Shinsaku

AU - Braguy, Justine

AU - Wang, Jian You

AU - Yoda, Akiyoshi

AU - Fiorilli, Valentina

AU - Takahashi, Ikuo

AU - Jamil, Muhammad

AU - Felemban, Abrar

AU - Miyazaki, Sho

AU - Mazzarella, Teresa

AU - Chen, Guan Ting Erica

AU - Shinozawa, Akihisa

AU - Balakrishna, Aparna

AU - Berqdar, Lamis

AU - Rajan, Chakravarty

AU - Ali, Shawkat

AU - Haider, Imran

AU - Sasaki, Yasuyuki

AU - Yajima, Shunsuke

AU - Akiyama, Kohki

AU - Lanfranco, Luisa

AU - Zurbriggen, Matias D.

AU - Nomura, Takahito

AU - Asami, Tadao

AU - Al-Babili, Salim

PY - 2022/11

Y1 - 2022/11

N2 - Strigolactones (SLs) are a plant hormone inhibiting shoot branching/tillering and a rhizospheric, chemical signal that triggers seed germination of the noxious root parasitic plant Striga and mediates symbiosis with beneficial arbuscular mycorrhizal fungi. Identifying specific roles of canonical and noncanonical SLs, the two SL subfamilies, is important for developing Striga-resistant cereals and for engineering plant architecture. Here, we report that rice mutants lacking canonical SLs do not show the shoot phenotypes known for SL-deficient plants, exhibiting only a delay in establishing arbuscular mycorrhizal symbiosis, but release exudates with a significantly decreased Striga seed-germinating activity. Blocking the biosynthesis of canonical SLs by TIS108, a specific enzyme inhibitor, significantly lowered Striga infestation without affecting rice growth. These results indicate that canonical SLs are not the determinant of shoot architecture and pave the way for increasing crop resistance by gene editing or chemical treatment.

AB - Strigolactones (SLs) are a plant hormone inhibiting shoot branching/tillering and a rhizospheric, chemical signal that triggers seed germination of the noxious root parasitic plant Striga and mediates symbiosis with beneficial arbuscular mycorrhizal fungi. Identifying specific roles of canonical and noncanonical SLs, the two SL subfamilies, is important for developing Striga-resistant cereals and for engineering plant architecture. Here, we report that rice mutants lacking canonical SLs do not show the shoot phenotypes known for SL-deficient plants, exhibiting only a delay in establishing arbuscular mycorrhizal symbiosis, but release exudates with a significantly decreased Striga seed-germinating activity. Blocking the biosynthesis of canonical SLs by TIS108, a specific enzyme inhibitor, significantly lowered Striga infestation without affecting rice growth. These results indicate that canonical SLs are not the determinant of shoot architecture and pave the way for increasing crop resistance by gene editing or chemical treatment.

UR - http://www.scopus.com/inward/record.url?scp=85141197439&partnerID=8YFLogxK

U2 - 10.1126/sciadv.add1278

DO - 10.1126/sciadv.add1278

M3 - Article

C2 - 36322663

AN - SCOPUS:85141197439

SN - 2375-2548

VL - 8

JO - SCIENCE ADVANCES

JF - SCIENCE ADVANCES

IS - 44

M1 - add1278

ER -

Canonical strigolactones are not the major determinant of tillering but important rhizospheric signals in rice

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