TY - JOUR
T1 - Cascade amplification strategy based on ultra-thin graphdiyne and CRISPR/Cas for real-time detection of tumor biomarker
AU - Xu, Jing
AU - Liu, Yinbing
AU - Huang, Ke Jing
AU - Wang, Renjie
AU - Li, Jiaqiang
N1 - KAUST Repository Item: Exported on 2023-05-09
Acknowledgements: The authors acknowledge financial support from the Talent Introduction Start-Up Foundation of Guangxi Minzu University (2021KJQD08), Natural Science Foundation of China (No.22074130), Key Scientific Research Projects of Henan Province (232102320040). This work was partly supported by the Florida Atlantic University startup grant. The authors also acknowledge the great support from the Analysis Testing Center of Xinyang Normal University for the material characterization.
PY - 2023/5/3
Y1 - 2023/5/3
N2 - As an important cancer markers, the sensitive, portable and highly specific detection of miRNA-141 is impendency. Currently, self-powered electrochemical sensors based on enzyme biofuel cells still difficult to be applied for rapid and sensitive detection for miRNAs. In this work, a sensitivity self-powered biosensor constructed by ultra-thin graphdiyne nanomaterials with good thermal stability and high electron mobility, combination of 3D DNA Walker mediated CRISPR/Cas12a cascade signal amplification strategy. In the linear range of 0.5–10000 fM, the detection limit is as low as 0.14 fM. Most importantly, the sensor platform can achieve real-time detection of miRNA-141 through smartphone. Finally, the relevant electrode reaction and signal amplification mechanism of sensing platform is explored, and the accurate determination of low content miRNA in complex biological samples is realized, which is expected to provide an effective method for the early diagnosis and prognosis of cancer patients.
AB - As an important cancer markers, the sensitive, portable and highly specific detection of miRNA-141 is impendency. Currently, self-powered electrochemical sensors based on enzyme biofuel cells still difficult to be applied for rapid and sensitive detection for miRNAs. In this work, a sensitivity self-powered biosensor constructed by ultra-thin graphdiyne nanomaterials with good thermal stability and high electron mobility, combination of 3D DNA Walker mediated CRISPR/Cas12a cascade signal amplification strategy. In the linear range of 0.5–10000 fM, the detection limit is as low as 0.14 fM. Most importantly, the sensor platform can achieve real-time detection of miRNA-141 through smartphone. Finally, the relevant electrode reaction and signal amplification mechanism of sensing platform is explored, and the accurate determination of low content miRNA in complex biological samples is realized, which is expected to provide an effective method for the early diagnosis and prognosis of cancer patients.
UR - http://hdl.handle.net/10754/691547
UR - https://linkinghub.elsevier.com/retrieve/pii/S1385894723019617
UR - http://www.scopus.com/inward/record.url?scp=85154608268&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2023.143230
DO - 10.1016/j.cej.2023.143230
M3 - Article
SN - 1385-8947
VL - 466
SP - 143230
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
ER -
