Cell refractive index tomography by digital holographic microscopy

Florian Charrière; Anca Marian; Frédéric Montfort; Jonas Kuehn; Tristan Colomb; Etienne Cuche; Pierre Marquet; Christian Depeursinge

doi:10.1364/OL.31.000178

Cell refractive index tomography by digital holographic microscopy

Florian Charrière^*, Anca Marian, Frédéric Montfort, Jonas Kuehn, Tristan Colomb, Etienne Cuche, Pierre Marquet, Christian Depeursinge

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

561 Scopus citations

Abstract

For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.

Original language	English (US)
Pages (from-to)	178-180
Number of pages	3
Journal	OPTICS LETTERS
Volume	31
Issue number	2
DOIs	https://doi.org/10.1364/OL.31.000178
State	Published - Jan 15 2006
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.31.000178

Cite this

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AU - Charrière, Florian

AU - Marian, Anca

AU - Montfort, Frédéric

AU - Kuehn, Jonas

AU - Colomb, Tristan

AU - Cuche, Etienne

AU - Marquet, Pierre

AU - Depeursinge, Christian

PY - 2006/1/15

Y1 - 2006/1/15

N2 - For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.

AB - For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.

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DO - 10.1364/OL.31.000178

M3 - Article

C2 - 16441022

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Cell refractive index tomography by digital holographic microscopy

Abstract

ASJC Scopus subject areas

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