Characterization of maize spermine synthase 1 (ZmSPMS1): Evidence for dimerization and intracellular location

Israel Maruri-López, Itzell E. Hernández-Sánchez, Alejandro Ferrando, Juan Carbonell, Juan Francisco Jiménez-Bremont*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Polyamines are ubiquitous positively charged metabolites that play an important role in wide fundamental cellular processes; because of their importance, the homeostasis of these amines is tightly regulated. Spermine synthase catalyzes the formation of polyamine spermine, which is necessary for growth and development in higher eukaryotes. Previously, we reported a stress inducible spermine synthase 1 (. ZmSPMS1) gene from maize. The ZmSPMS1 enzyme differs from their dicot orthologous by a C-terminal extension, which contains a degradation PEST sequence involved in its turnover. Herein, we demonstrate that ZmSPMS1 protein interacts with itself in split yeast two-hybrid (Y2H) assays. A Bimolecular Fluorescence Complementation (BiFC) assay revealed that ZmSPMS1 homodimer has a cytoplasmic localization. In order to gain a better understanding about ZmSPMS1 interaction, two deletion constructs of ZmSPMS1 protein were obtained. The δN-ZmSPMS1 version, where the first 74 N-terminal amino acids were eliminated, showed reduced capability of dimer formation, whereas the δC-ZmSPMS1 version, lacking the last 40 C-terminal residues, dramatically abated the ZmSPMS1-ZmSPMS1 protein interaction. Recombinant protein expression in Escherichia coli of ZmSPMS1 derived versions revealed that deletion of its N-terminal domain affected the spermine biosynthesis, whereas C-terminal ZmSPMS1 truncated version fail to generate this polyamine. These data suggest that N- and C-terminal domains of ZmSPMS1 play a role in a functional homodimer.

Original languageEnglish (US)
Pages (from-to)264-271
Number of pages8
JournalPlant Physiology and Biochemistry
StatePublished - Dec 1 2015


  • Bimolecular fluorescence complementation
  • C-terminal
  • Homodimer
  • Spermine synthase
  • Spermine synthesis
  • Subcellular localization
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science


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