TY - JOUR
T1 - Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.)
AU - Banguera Hinestroza, Eulalia
AU - Saenz Agudelo, Pablo
AU - Bayer, Till
AU - Berumen, Michael L.
AU - Voolstra, Christian R.
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2013/1/9
Y1 - 2013/1/9
N2 - A total of one hundred microsatellites loci were selected from the draft genome of Stylophora pistillata and evaluated in previously characterized samples of Stylophora cf pistillata from the Red Sea. 17 loci were amplified successfully and tested in 24 individuals from samples belonging to a single population from the central region of the Red Sea. The number of alleles ranged from 3 to 15 alleles per locus, while observed heterozygosity ranged from 0. 292 to 0. 95. Six of these loci showed significant deviations from Hardy-Weinberg equilibrium (HWE) expectations, and 4/136 paired loci comparisons suggested linkage disequilibrium after Bonferroni corrections. After excluding loci with significant HWE deviation and evidence of null alleles, average genetic diversity over loci in the population studied (N = 24, Nloci = 11) was 0. 701 ± 0. 380. This indicates that these loci can be used effectively to evaluate genetic diversity and undertake population genetics studies in Stylophora sp. populations. 2013 The Author(s).
AB - A total of one hundred microsatellites loci were selected from the draft genome of Stylophora pistillata and evaluated in previously characterized samples of Stylophora cf pistillata from the Red Sea. 17 loci were amplified successfully and tested in 24 individuals from samples belonging to a single population from the central region of the Red Sea. The number of alleles ranged from 3 to 15 alleles per locus, while observed heterozygosity ranged from 0. 292 to 0. 95. Six of these loci showed significant deviations from Hardy-Weinberg equilibrium (HWE) expectations, and 4/136 paired loci comparisons suggested linkage disequilibrium after Bonferroni corrections. After excluding loci with significant HWE deviation and evidence of null alleles, average genetic diversity over loci in the population studied (N = 24, Nloci = 11) was 0. 701 ± 0. 380. This indicates that these loci can be used effectively to evaluate genetic diversity and undertake population genetics studies in Stylophora sp. populations. 2013 The Author(s).
UR - http://hdl.handle.net/10754/334649
UR - http://link.springer.com/10.1007/s12686-012-9852-x
UR - http://www.scopus.com/inward/record.url?scp=84876824583&partnerID=8YFLogxK
U2 - 10.1007/s12686-012-9852-x
DO - 10.1007/s12686-012-9852-x
M3 - Article
SN - 1877-7252
VL - 5
SP - 561
EP - 563
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -