TY - JOUR
T1 - Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell
AU - Zhang, Gen
AU - He, Li-sheng
AU - Wong, Yue-Him
AU - Xu, Ying
AU - Zhang, Yu
AU - Qian, Pei-Yuan
N1 - KAUST Repository Item: Exported on 2021-11-01
Acknowledgements: This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and grants (GRF661611, GRF662413) from the Research Grants Council of the Hong Kong Special Administrative Region to PYQ as well as a grant from the National Natural Science Foundation of China (31460092) to LSH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2015
Y1 - 2015
N2 - As typical biofoulers, barnacles possess hard shells and cause serious biofouling problems. In this study, we analyzed the protein component of the barnacle Amphibalanus (= Balanus) amphitrite shell using gel-based proteomics. The results revealed 52 proteins in the A. Amphitrite shell. Among them, 40 proteins were categorized into 11 functional groups based on KOG database, and the remaining 12 proteins were unknown. Besides the known proteins in barnacle shell (SIPC, carbonic anhydrase and acidic acid matrix protein), we also identified chorion peroxidase, C-type lectin-like domains, serine proteases and proteinase inhibitor proteins in the A. Amphitrite shell. The sequences of these proteins were characterized and their potential functions were discussed. Histology and DAPI staining revealed living cells in the shell, which might secrete the shell proteins identified in this study.
AB - As typical biofoulers, barnacles possess hard shells and cause serious biofouling problems. In this study, we analyzed the protein component of the barnacle Amphibalanus (= Balanus) amphitrite shell using gel-based proteomics. The results revealed 52 proteins in the A. Amphitrite shell. Among them, 40 proteins were categorized into 11 functional groups based on KOG database, and the remaining 12 proteins were unknown. Besides the known proteins in barnacle shell (SIPC, carbonic anhydrase and acidic acid matrix protein), we also identified chorion peroxidase, C-type lectin-like domains, serine proteases and proteinase inhibitor proteins in the A. Amphitrite shell. The sequences of these proteins were characterized and their potential functions were discussed. Histology and DAPI staining revealed living cells in the shell, which might secrete the shell proteins identified in this study.
UR - http://hdl.handle.net/10754/672997
UR - https://dx.plos.org/10.1371/journal.pone.0133866
UR - http://www.scopus.com/inward/record.url?scp=84941924473&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0133866
DO - 10.1371/journal.pone.0133866
M3 - Article
C2 - 26222041
SN - 1932-6203
VL - 10
SP - e0133866
JO - PLOS ONE
JF - PLOS ONE
IS - 7
ER -