Chiral electrochromatography with a 'moulded' rigid monolithic capillary column

Eric C. Peters; Kevin Lewandowski; Miroslav Petro; Frantisek Svec; Jean M.J. Fréchet

doi:10.1039/a708968f

Chiral electrochromatography with a 'moulded' rigid monolithic capillary column

Eric C. Peters^*, Kevin Lewandowski, Miroslav Petro, Frantisek Svec, Jean M.J. Fréchet

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

129 Scopus citations

Abstract

Several monolithic chiral stationary phases for reversed-phase electrochromatography have been prepared within the confines of untreated fused silica capillaries by the direct copolymerization of the chiral monomer 2-hydroxyethyl methacrylate (N-L-valine-3,5-dimethylanilide) carbamate with ethylene dimethacrylate, 2-acrylamido-2-methyl-1-propanesulfonic acid and butyl or glycidyl methacrylate in the presence of a porogenic solvent. The hydrophilicity of the stationary phase, which may be enhanced further by the hydrolysis of the epoxide functionalities of the glycidyl methacrylate moieties within the monolith, was found to have a pronounced effect on the enantioseparation. Using the most hydrophilic monolithic capillary column and optimized elution conditions, a separation of N-(3,5-dinitrobenzoyl)leucine diallylamide enantiomers with an efficiency of 61000 plates m^-1 and a resolution of 2.0 was achieved.

Original language	English (US)
Pages (from-to)	83-86
Number of pages	4
Journal	Analytical Communications
Volume	35
Issue number	3
DOIs	https://doi.org/10.1039/a708968f
State	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1039/a708968f

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title = "Chiral electrochromatography with a 'moulded' rigid monolithic capillary column",

abstract = "Several monolithic chiral stationary phases for reversed-phase electrochromatography have been prepared within the confines of untreated fused silica capillaries by the direct copolymerization of the chiral monomer 2-hydroxyethyl methacrylate (N-L-valine-3,5-dimethylanilide) carbamate with ethylene dimethacrylate, 2-acrylamido-2-methyl-1-propanesulfonic acid and butyl or glycidyl methacrylate in the presence of a porogenic solvent. The hydrophilicity of the stationary phase, which may be enhanced further by the hydrolysis of the epoxide functionalities of the glycidyl methacrylate moieties within the monolith, was found to have a pronounced effect on the enantioseparation. Using the most hydrophilic monolithic capillary column and optimized elution conditions, a separation of N-(3,5-dinitrobenzoyl)leucine diallylamide enantiomers with an efficiency of 61000 plates m-1 and a resolution of 2.0 was achieved.",

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T1 - Chiral electrochromatography with a 'moulded' rigid monolithic capillary column

AU - Peters, Eric C.

AU - Lewandowski, Kevin

AU - Petro, Miroslav

AU - Svec, Frantisek

AU - Fréchet, Jean M.J.

PY - 1998

Y1 - 1998

N2 - Several monolithic chiral stationary phases for reversed-phase electrochromatography have been prepared within the confines of untreated fused silica capillaries by the direct copolymerization of the chiral monomer 2-hydroxyethyl methacrylate (N-L-valine-3,5-dimethylanilide) carbamate with ethylene dimethacrylate, 2-acrylamido-2-methyl-1-propanesulfonic acid and butyl or glycidyl methacrylate in the presence of a porogenic solvent. The hydrophilicity of the stationary phase, which may be enhanced further by the hydrolysis of the epoxide functionalities of the glycidyl methacrylate moieties within the monolith, was found to have a pronounced effect on the enantioseparation. Using the most hydrophilic monolithic capillary column and optimized elution conditions, a separation of N-(3,5-dinitrobenzoyl)leucine diallylamide enantiomers with an efficiency of 61000 plates m-1 and a resolution of 2.0 was achieved.

AB - Several monolithic chiral stationary phases for reversed-phase electrochromatography have been prepared within the confines of untreated fused silica capillaries by the direct copolymerization of the chiral monomer 2-hydroxyethyl methacrylate (N-L-valine-3,5-dimethylanilide) carbamate with ethylene dimethacrylate, 2-acrylamido-2-methyl-1-propanesulfonic acid and butyl or glycidyl methacrylate in the presence of a porogenic solvent. The hydrophilicity of the stationary phase, which may be enhanced further by the hydrolysis of the epoxide functionalities of the glycidyl methacrylate moieties within the monolith, was found to have a pronounced effect on the enantioseparation. Using the most hydrophilic monolithic capillary column and optimized elution conditions, a separation of N-(3,5-dinitrobenzoyl)leucine diallylamide enantiomers with an efficiency of 61000 plates m-1 and a resolution of 2.0 was achieved.

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JO - Analytical Communications

JF - Analytical Communications

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Chiral electrochromatography with a 'moulded' rigid monolithic capillary column

Abstract

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