TY - JOUR
T1 - Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos
AU - Oelgeschläger, Michael
AU - Kuroda, Hiroki
AU - Reversade, Bruno
AU - De Robertis, E. M.
N1 - Generated from Scopus record by KAUST IRTS on 2023-02-15
PY - 2003/2/1
Y1 - 2003/2/1
N2 - We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.
AB - We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.
UR - https://linkinghub.elsevier.com/retrieve/pii/S1534580702004045
UR - http://www.scopus.com/inward/record.url?scp=0037321789&partnerID=8YFLogxK
U2 - 10.1016/S1534-5807(02)00404-5
DO - 10.1016/S1534-5807(02)00404-5
M3 - Article
SN - 1534-5807
VL - 4
SP - 219
EP - 230
JO - Developmental Cell
JF - Developmental Cell
IS - 2
ER -