Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA

Thennarasu Sugumar; M. Harishankar; G. Dhinakar Raj

doi:10.1111/j.1744-313X.2011.01075.x

Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA

Thennarasu Sugumar, M. Harishankar, G. Dhinakar Raj

Research output: Contribution to journal › Article › peer-review

Abstract

Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.

Original language	English (US)
Pages (from-to)	203-206
Number of pages	4
Journal	International Journal of Immunogenetics
Volume	39
Issue number	3
DOIs	https://doi.org/10.1111/j.1744-313X.2011.01075.x
State	Published - Dec 12 2011
Externally published	Yes

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title = "Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA",

abstract = "Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.",

author = "Thennarasu Sugumar and M. Harishankar and {Dhinakar Raj}, G.",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors thank the Department of Biotechnology (DBT), Government of India, for financial assistance to this Project. ST and MHS were SRFs who worked in this project.",

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AU - Harishankar, M.

AU - Dhinakar Raj, G.

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AB - Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.

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JO - International Journal of Immunogenetics

JF - International Journal of Immunogenetics

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Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA

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