Abstract
Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.
Original language | English (US) |
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Pages (from-to) | 203-206 |
Number of pages | 4 |
Journal | International Journal of Immunogenetics |
Volume | 39 |
Issue number | 3 |
DOIs | |
State | Published - Dec 12 2011 |
Externally published | Yes |