@article{49158d2a5ace4df9ab0424859d488772,
title = "Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii",
abstract = "The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.",
author = "Guifang Lin and Hui Chen and Bin Tian and Sehgal, {Sunish K.} and Lovepreet Singh and Jingzhong Xie and Nidhi Rawat and Philomin Juliana and Narinder Singh and Sandesh Shrestha and Wilson, {Duane L.} and Hannah Shult and Hyeonju Lee and Schoen, {Adam William} and Tiwari, {Vijay K.} and Singh, {Ravi P.} and Guttieri, {Mary J.} and Trick, {Harold N.} and Jesse Poland and Bowden, {Robert L.} and Guihua Bai and Bikram Gill and Sanzhen Liu",
note = "KAUST Repository Item: Exported on 2022-06-03 Acknowledgements: Funding was provided by the Kansas Wheat Commission award (no. KWC2020-08) to S.L.; the NSF award (no. 1238313) to B.G.; and the NSF award (no. 1822162) to J.P. Additional funding to support this project includes the USDA NIFA awards (nos. 2018-67013-28511 and 2021-67013-35724) to S.L., the NSF awards (nos. 1741090 and 2011500) to S.L., the NSF award (no. 1943155) to N.R. and V.K.T., and the USDA NIFA awards (nos. 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T. We thank Dr. Christopher Toomajian for the discussion, Dr. Alina Akhunova from the Integrative Genomics Facility at Kansas State University for preparation of RNA-Seq libraries, John Raupp for maintaining and sharing Ae. tauschii accessions, Shuangye Wu for DNA extraction and genotyping, Dr. Hongliang Wang for Lr42 genotyping of wheat populations, Andrew Fan Bai for peeling Ae. tauschii seeds, and Taylor Schulden for rust inoculation and phenotyping. This is contribution 20-131-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas.",
year = "2022",
month = jun,
day = "1",
doi = "10.1038/s41467-022-30784-9",
language = "English (US)",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Research",
number = "1",
}