Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles

Dan Cojoc; Enrico Ferrari; Valeria Garbin; Enzo Di Fabrizio; Heinz Amenitsch; Michel Rappolt; Barbara Sartori; Christian Riekel; Manfred Burghammer

doi:10.1117/12.680643

Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles

Dan Cojoc^*, Enrico Ferrari, Valeria Garbin, Enzo Di Fabrizio, Heinz Amenitsch, Michel Rappolt, Barbara Sartori, Christian Riekel, Manfred Burghammer

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

We present and discuss a new experimental setup to perform small angle X-ray scattering and diffraction (SAXSD) of localized liposome colloidal microparticles. A home-built inverted infrared laser tweezers microscope is used to trap, manipulate and aggregate micron-scale liposome particles at single locations inside a 100 microns glass capillary. The micro-focused X-ray and the laser beams are aligned to intersect each other perpendiculary, allowing to associate the X-ray diffraction signal to the micron-sized region of interest inside the capillary. Throughout the laser tweezer setup, using diffractive optical elements implemented on a spatial light modulator, we are able to manipulate small aggregates of colloidal particles (liposomes) and fix them in the optical path of the X-ray beam. We present and discuss first scattering and diffraction experiments on phospholipid liposomes, at the ID 13 microfocus beamline of the European Synchrotron Radiation Facility (ESRF The results demonstrate that we can push the limit of measurable cluster size close to a single liposome.

Original language	English (US)
Title of host publication	Optical Trapping and Optical Micromanipulation III
DOIs	https://doi.org/10.1117/12.680643
State	Published - 2006
Externally published	Yes
Event	Optical Trapping and Optical Micromanipulation III - San Diego, CA, United States Duration: Aug 13 2006 → Aug 17 2006

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6326
ISSN (Print)	0277-786X

Other

Other	Optical Trapping and Optical Micromanipulation III
Country/Territory	United States
City	San Diego, CA
Period	08/13/06 → 08/17/06

Keywords

Optical tweezers
Phospholipids
Single biological entity
X-ray microdiffraction

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.680643

Cite this

Cojoc, D., Ferrari, E., Garbin, V., Di Fabrizio, E., Amenitsch, H., Rappolt, M., Sartori, B., Riekel, C., & Burghammer, M. (2006). Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles. In Optical Trapping and Optical Micromanipulation III Article 63261M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6326). https://doi.org/10.1117/12.680643

@inproceedings{edb0dc83411f4f7190431e002a11e05c,

title = "Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles",

abstract = "We present and discuss a new experimental setup to perform small angle X-ray scattering and diffraction (SAXSD) of localized liposome colloidal microparticles. A home-built inverted infrared laser tweezers microscope is used to trap, manipulate and aggregate micron-scale liposome particles at single locations inside a 100 microns glass capillary. The micro-focused X-ray and the laser beams are aligned to intersect each other perpendiculary, allowing to associate the X-ray diffraction signal to the micron-sized region of interest inside the capillary. Throughout the laser tweezer setup, using diffractive optical elements implemented on a spatial light modulator, we are able to manipulate small aggregates of colloidal particles (liposomes) and fix them in the optical path of the X-ray beam. We present and discuss first scattering and diffraction experiments on phospholipid liposomes, at the ID 13 microfocus beamline of the European Synchrotron Radiation Facility (ESRF The results demonstrate that we can push the limit of measurable cluster size close to a single liposome.",

keywords = "Optical tweezers, Phospholipids, Single biological entity, X-ray microdiffraction",

author = "Dan Cojoc and Enrico Ferrari and Valeria Garbin and {Di Fabrizio}, Enzo and Heinz Amenitsch and Michel Rappolt and Barbara Sartori and Christian Riekel and Manfred Burghammer",

year = "2006",

doi = "10.1117/12.680643",

language = "English (US)",

isbn = "0819464058",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Optical Trapping and Optical Micromanipulation III",

note = "Optical Trapping and Optical Micromanipulation III ; Conference date: 13-08-2006 Through 17-08-2006",

}

Cojoc, D, Ferrari, E, Garbin, V, Di Fabrizio, E, Amenitsch, H, Rappolt, M, Sartori, B, Riekel, C & Burghammer, M 2006, Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles. in Optical Trapping and Optical Micromanipulation III., 63261M, Proceedings of SPIE - The International Society for Optical Engineering, vol. 6326, Optical Trapping and Optical Micromanipulation III, San Diego, CA, United States, 08/13/06. https://doi.org/10.1117/12.680643

Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles. / Cojoc, Dan; Ferrari, Enrico; Garbin, Valeria et al.
Optical Trapping and Optical Micromanipulation III. 2006. 63261M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6326).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Ferrari, Enrico

AU - Garbin, Valeria

AU - Di Fabrizio, Enzo

AU - Amenitsch, Heinz

AU - Rappolt, Michel

AU - Sartori, Barbara

AU - Riekel, Christian

AU - Burghammer, Manfred

PY - 2006

Y1 - 2006

N2 - We present and discuss a new experimental setup to perform small angle X-ray scattering and diffraction (SAXSD) of localized liposome colloidal microparticles. A home-built inverted infrared laser tweezers microscope is used to trap, manipulate and aggregate micron-scale liposome particles at single locations inside a 100 microns glass capillary. The micro-focused X-ray and the laser beams are aligned to intersect each other perpendiculary, allowing to associate the X-ray diffraction signal to the micron-sized region of interest inside the capillary. Throughout the laser tweezer setup, using diffractive optical elements implemented on a spatial light modulator, we are able to manipulate small aggregates of colloidal particles (liposomes) and fix them in the optical path of the X-ray beam. We present and discuss first scattering and diffraction experiments on phospholipid liposomes, at the ID 13 microfocus beamline of the European Synchrotron Radiation Facility (ESRF The results demonstrate that we can push the limit of measurable cluster size close to a single liposome.

AB - We present and discuss a new experimental setup to perform small angle X-ray scattering and diffraction (SAXSD) of localized liposome colloidal microparticles. A home-built inverted infrared laser tweezers microscope is used to trap, manipulate and aggregate micron-scale liposome particles at single locations inside a 100 microns glass capillary. The micro-focused X-ray and the laser beams are aligned to intersect each other perpendiculary, allowing to associate the X-ray diffraction signal to the micron-sized region of interest inside the capillary. Throughout the laser tweezer setup, using diffractive optical elements implemented on a spatial light modulator, we are able to manipulate small aggregates of colloidal particles (liposomes) and fix them in the optical path of the X-ray beam. We present and discuss first scattering and diffraction experiments on phospholipid liposomes, at the ID 13 microfocus beamline of the European Synchrotron Radiation Facility (ESRF The results demonstrate that we can push the limit of measurable cluster size close to a single liposome.

KW - Optical tweezers

KW - Phospholipids

KW - Single biological entity

KW - X-ray microdiffraction

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SN - 0819464058

SN - 9780819464057

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Optical Trapping and Optical Micromanipulation III

T2 - Optical Trapping and Optical Micromanipulation III

Y2 - 13 August 2006 through 17 August 2006

ER -

Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles

Abstract

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Keywords

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