TY - JOUR
T1 - Comparison of two homogeneous HDL cholesterol methods in a large population study
AU - Chittamma, Anchalee
AU - Roberts, William L.
AU - Sritara, Piyamitr
AU - Cheepudomwit, Sayan
AU - Suriyawongpaisal, Paibul
AU - Lolekha, Porntip H.
N1 - Generated from Scopus record by KAUST IRTS on 2023-09-20
PY - 2004/9/1
Y1 - 2004/9/1
N2 - Objectives: High-density lipoprotein cholesterol (HDL-C) is an independent risk factor for coronary heart disease. Data are lacking on the performance of homogeneous methods using a large number of samples. Design and methods: We compared the performance of two HDL-C direct assays, the Dimension RxL (the Dade method) and the COBAS INTEGRA (the Roche method), for population screening. Performance was assessed using 4214 sera obtained from the International Collaborative Study on Atherosclerosis and Stroke In Asia (InterASIA) participants. Results: The method comparison results demonstrated that both methods were highly correlated (r = 0.972). Deming regression analysis showed a slope of 1.009 ± 0.007, an intercept of 0.048 ± 0.009 and a S y/x of 0.08. The means were 1.29 ± 0.33 and 1.23 ± 0.33 mmol/l for the Roche and Dade methods, respectively, and the range of observed values were 0.30-3.05 and 0.19-2.86 mmol/l, respectively. The 95% confidence interval for the mean of the method differences was -0.10 to 0.22 mmol/l. Percentages of low (≥1.55 mmol/l), normal (1.03-1.54 mmol/l), and high risk (
AB - Objectives: High-density lipoprotein cholesterol (HDL-C) is an independent risk factor for coronary heart disease. Data are lacking on the performance of homogeneous methods using a large number of samples. Design and methods: We compared the performance of two HDL-C direct assays, the Dimension RxL (the Dade method) and the COBAS INTEGRA (the Roche method), for population screening. Performance was assessed using 4214 sera obtained from the International Collaborative Study on Atherosclerosis and Stroke In Asia (InterASIA) participants. Results: The method comparison results demonstrated that both methods were highly correlated (r = 0.972). Deming regression analysis showed a slope of 1.009 ± 0.007, an intercept of 0.048 ± 0.009 and a S y/x of 0.08. The means were 1.29 ± 0.33 and 1.23 ± 0.33 mmol/l for the Roche and Dade methods, respectively, and the range of observed values were 0.30-3.05 and 0.19-2.86 mmol/l, respectively. The 95% confidence interval for the mean of the method differences was -0.10 to 0.22 mmol/l. Percentages of low (≥1.55 mmol/l), normal (1.03-1.54 mmol/l), and high risk (
UR - https://linkinghub.elsevier.com/retrieve/pii/S0009912004001213
UR - http://www.scopus.com/inward/record.url?scp=4344673541&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2004.05.001
DO - 10.1016/j.clinbiochem.2004.05.001
M3 - Article
SN - 0009-9120
VL - 37
SP - 745
EP - 749
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 9
ER -