TY - JOUR
T1 - Complete Genome Sequence of Cellulomonas sp. JZ18, a Root Endophytic Bacterium Isolated from the Perennial Desert Tussock-Grass Panicum turgidum
AU - Eida, Abdul Aziz
AU - Bougouffa, Salim
AU - Alam, Intikhab
AU - Hirt, Heribert
AU - Saad, Maged
N1 - KAUST Repository Item: Exported on 2021-03-10
Acknowledgements: The work presented is part of the DARWIN21 project (http://www.darwin21.org/), with the objective to improve sustainable agriculture on arid lands by exploiting microbes isolated from pioneer desert plants that are able to survive in extreme environmental conditions. The authors would thank all members of Hirt lab, CDA management team and the Bioscience Core Labs in KAUST for the technical assistance and for their help in many aspects of this work.
PY - 2021/3/8
Y1 - 2021/3/8
N2 - Cellulomonas sp. JZ18 is a gram-positive, rod shaped bacterium that was previously isolated from the root endosphere of the perennial desert tussock-grass Panicum turgidum. Genome coverage of PacBio sequencing was approximately 199X. Genome assembly generated a single chromosome of 7,421,843 base pairs with a guanine-cytosine (GC) content of 75.60% with 3240 protein coding sequences, 361 pseudo genes, three ribosomal RNA operons, three non-coding RNAs and 45 transfer RNAs. Comparison of JZ18′s genome with type strains from the same genus, using digital DNA–DNA hybridization and average nucleotide identity calculations, revealed that JZ18 might potentially belong to a new species. Functional analysis revealed the presence of genes that may complement previously observed biochemical and plant phenotypes. Furthermore, the presence of a number of enzymes could be of potential use in industrial processes as biocatalysts. Genome sequencing and analysis, coupled with comparative genomics, of endophytic bacteria for their potential plant growth promoting activities under different soil conditions will accelerate the knowledge and applications of biostimulants in sustainable agriculture.
AB - Cellulomonas sp. JZ18 is a gram-positive, rod shaped bacterium that was previously isolated from the root endosphere of the perennial desert tussock-grass Panicum turgidum. Genome coverage of PacBio sequencing was approximately 199X. Genome assembly generated a single chromosome of 7,421,843 base pairs with a guanine-cytosine (GC) content of 75.60% with 3240 protein coding sequences, 361 pseudo genes, three ribosomal RNA operons, three non-coding RNAs and 45 transfer RNAs. Comparison of JZ18′s genome with type strains from the same genus, using digital DNA–DNA hybridization and average nucleotide identity calculations, revealed that JZ18 might potentially belong to a new species. Functional analysis revealed the presence of genes that may complement previously observed biochemical and plant phenotypes. Furthermore, the presence of a number of enzymes could be of potential use in industrial processes as biocatalysts. Genome sequencing and analysis, coupled with comparative genomics, of endophytic bacteria for their potential plant growth promoting activities under different soil conditions will accelerate the knowledge and applications of biostimulants in sustainable agriculture.
UR - http://hdl.handle.net/10754/667982
UR - http://link.springer.com/10.1007/s00284-021-02429-5
U2 - 10.1007/s00284-021-02429-5
DO - 10.1007/s00284-021-02429-5
M3 - Article
C2 - 33683416
SN - 0343-8651
JO - Current Microbiology
JF - Current Microbiology
ER -