Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes

Jon Reinders, Brande B.H. Wulff, Marie Mirouze, Arturo Marí-Ordóñez, Mélanie Dapp, Wilfried Rozhon, Etienne Bucher, Grégory Theiler, Jerzy Paszkowski

Research output: Contribution to journalArticlepeer-review

362 Scopus citations

Abstract

Transgenerational epigenetic inheritance has been defined by the study of relatively few loci. We examined a population of recombinant inbred lines with epigenetically mosaic chromosomes consisting of wild-type and CG methylation-depleted segments (epiRILs). Surprisingly, transposons that were immobile in the parental lines displayed stochastic movement in 28% of the epiRILs. Although analysis after eight generations of inbreeding, supported by genome-wide DNA methylation profiling, identified recombined parental chromosomal segments, these were interspersed with unexpectedly high frequencies of nonparental methylation polymorphism. Hence, epigenetic inheritance in hybrids derived from parents with divergent epigenomes permits long-lasting epi-allelic interactions that violate Mendelian expectations. Such persistently "unstable" epigenetic states complicate linkage-based epigenomic mapping. Thus, future epigenomic analyses should consider possible genetic instabilities and alternative mapping strategies. © 2009 by Cold Spring Harbor Laboratory Press.
Original languageEnglish (US)
Pages (from-to)939-950
Number of pages12
JournalGenes and Development
Volume23
Issue number8
DOIs
StatePublished - Apr 15 2009
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine

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