Confocal imaging of ionised calcium in living plant cells

D. A. Williams; S. H. Cody; C. A. Gehring; R. W. Parish; P. J. Harris

doi:10.1016/0143-4160(90)90006-G

Confocal imaging of ionised calcium in living plant cells

D. A. Williams^*, S. H. Cody, C. A. Gehring, R. W. Parish, P. J. Harris

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Laser-scanning confocal microscopy has been used in conjunction with Fluo-3, a highly fluorescent visible wavelength probe for Ca²⁺, to visualise Ca²⁺-dynamics in the function of living plant cells. This combination has overcome many of the problems that have limited the use of fluorescence imaging techniques in the study of the role of cations (Ca²⁺ and H⁺) in plant cell physiology and enables these processes to be studied in single cells within intact plant tissue preparations. Maize coleoptiles respond to application of ionophores and plant growth hormones with elevations in cytosolic Ca²⁺ that can be resolved with a high degree of spatial resolution and can be interpreted quantitatively.

Original language	English (US)
Pages (from-to)	291-297
Number of pages	7
Journal	Cell Calcium
Volume	11
Issue number	4
DOIs	https://doi.org/10.1016/0143-4160(90)90006-G
State	Published - Apr 1990
Externally published	Yes

ASJC Scopus subject areas

Physiology
Molecular Biology
Cell Biology

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title = "Confocal imaging of ionised calcium in living plant cells",

abstract = "Laser-scanning confocal microscopy has been used in conjunction with Fluo-3, a highly fluorescent visible wavelength probe for Ca2+, to visualise Ca2+-dynamics in the function of living plant cells. This combination has overcome many of the problems that have limited the use of fluorescence imaging techniques in the study of the role of cations (Ca2+ and H+) in plant cell physiology and enables these processes to be studied in single cells within intact plant tissue preparations. Maize coleoptiles respond to application of ionophores and plant growth hormones with elevations in cytosolic Ca2+ that can be resolved with a high degree of spatial resolution and can be interpreted quantitatively.",

author = "Williams, {D. A.} and Cody, {S. H.} and Gehring, {C. A.} and Parish, {R. W.} and Harris, {P. J.}",

year = "1990",

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doi = "10.1016/0143-4160(90)90006-G",

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T1 - Confocal imaging of ionised calcium in living plant cells

AU - Williams, D. A.

AU - Cody, S. H.

AU - Gehring, C. A.

AU - Parish, R. W.

AU - Harris, P. J.

PY - 1990/4

Y1 - 1990/4

N2 - Laser-scanning confocal microscopy has been used in conjunction with Fluo-3, a highly fluorescent visible wavelength probe for Ca2+, to visualise Ca2+-dynamics in the function of living plant cells. This combination has overcome many of the problems that have limited the use of fluorescence imaging techniques in the study of the role of cations (Ca2+ and H+) in plant cell physiology and enables these processes to be studied in single cells within intact plant tissue preparations. Maize coleoptiles respond to application of ionophores and plant growth hormones with elevations in cytosolic Ca2+ that can be resolved with a high degree of spatial resolution and can be interpreted quantitatively.

AB - Laser-scanning confocal microscopy has been used in conjunction with Fluo-3, a highly fluorescent visible wavelength probe for Ca2+, to visualise Ca2+-dynamics in the function of living plant cells. This combination has overcome many of the problems that have limited the use of fluorescence imaging techniques in the study of the role of cations (Ca2+ and H+) in plant cell physiology and enables these processes to be studied in single cells within intact plant tissue preparations. Maize coleoptiles respond to application of ionophores and plant growth hormones with elevations in cytosolic Ca2+ that can be resolved with a high degree of spatial resolution and can be interpreted quantitatively.

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VL - 11

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JO - Cell Calcium

JF - Cell Calcium

IS - 4

ER -

Confocal imaging of ionised calcium in living plant cells

Abstract

ASJC Scopus subject areas

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