Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability

Silvia Nappini; Massimo Bonini; Francesca Baldelli Bombelli; Francesco Pineider; Claudio Sangregorio; Piero Baglioni; Bengt Nordèn

doi:10.1039/c0sm00789g

Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability

Silvia Nappini, Massimo Bonini, Francesca Baldelli Bombelli, Francesco Pineider, Claudio Sangregorio, Piero Baglioni, Bengt Nordèn

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Abstract

The paper describes the effect of a low-frequency alternating magnetic field (LF-AMF) on the permeability and release properties of large (LUVs) and giant (GUVs) unilamellar vesicles loaded with citrate coated cobalt ferrite nanoparticles (NPs). The citrate shell allows a high loading of NPs in lipid vesicles without modifying their magnetic properties. The increase of magnetic LUVs permeability upon exposure to LF-AMF has been evaluated as the fluorescence self-quenching of carboxyfluorescein (CF) entrapped inside the liposome aqueous pool. Liposome leakage has been monitored as a function of field frequency, time exposure and concentration of the citrate coated NPs. Confocal Laser Scanning Microscopy (CLSM) experiments performed on magnetic GUVs labeled with the fluorescent probe DiIC18 and loaded with Alexa 488-C5-maleimide fluorescent dye provided insights on the release mechanism induced by LF-AMF. The results show that LF-AMF strongly affects vesicles permeability, suggesting the formation of pores in the lipid bilayer due to both hyperthermic effects and nanoparticle oscillations in the vesicles pool at the applied frequency. The behaviour of these magnetic vesicles in the presence of LF-AMF makes this system a good candidate for controlled drug delivery. © 2011 The Royal Society of Chemistry.

Original language	English (US)
Pages (from-to)	1025-1037
Number of pages	13
Journal	Soft Matter
Volume	7
Issue number	3
DOIs	https://doi.org/10.1039/c0sm00789g
State	Published - 2011
Externally published	Yes

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title = "Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability",

abstract = "The paper describes the effect of a low-frequency alternating magnetic field (LF-AMF) on the permeability and release properties of large (LUVs) and giant (GUVs) unilamellar vesicles loaded with citrate coated cobalt ferrite nanoparticles (NPs). The citrate shell allows a high loading of NPs in lipid vesicles without modifying their magnetic properties. The increase of magnetic LUVs permeability upon exposure to LF-AMF has been evaluated as the fluorescence self-quenching of carboxyfluorescein (CF) entrapped inside the liposome aqueous pool. Liposome leakage has been monitored as a function of field frequency, time exposure and concentration of the citrate coated NPs. Confocal Laser Scanning Microscopy (CLSM) experiments performed on magnetic GUVs labeled with the fluorescent probe DiIC18 and loaded with Alexa 488-C5-maleimide fluorescent dye provided insights on the release mechanism induced by LF-AMF. The results show that LF-AMF strongly affects vesicles permeability, suggesting the formation of pores in the lipid bilayer due to both hyperthermic effects and nanoparticle oscillations in the vesicles pool at the applied frequency. The behaviour of these magnetic vesicles in the presence of LF-AMF makes this system a good candidate for controlled drug delivery. {\textcopyright} 2011 The Royal Society of Chemistry.",

author = "Silvia Nappini and Massimo Bonini and Bombelli, {Francesca Baldelli} and Francesco Pineider and Claudio Sangregorio and Piero Baglioni and Bengt Nord{\`e}n",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Financial support from CSGI and MIUR-PRIN 2008 is acknowledged. We thank Debora Berti and Al Kayal Tamer for their helpful advices for GUVs preparation and Professor R. Udisti and F. Rugi for ICP-OES experiments. Katarina Edwards is also gratefully acknowledged for Cryo-TEM images of magnetoliposomes and Dr C. Innocenti for scientific discussion. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr Tore Eriksson is acknowledged for building the alternating magnetic field generator. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2011",

doi = "10.1039/c0sm00789g",

language = "English (US)",

volume = "7",

pages = "1025--1037",

journal = "Soft Matter",

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publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability

AU - Nappini, Silvia

AU - Bonini, Massimo

AU - Bombelli, Francesca Baldelli

AU - Pineider, Francesco

AU - Sangregorio, Claudio

AU - Baglioni, Piero

AU - Nordèn, Bengt

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Financial support from CSGI and MIUR-PRIN 2008 is acknowledged. We thank Debora Berti and Al Kayal Tamer for their helpful advices for GUVs preparation and Professor R. Udisti and F. Rugi for ICP-OES experiments. Katarina Edwards is also gratefully acknowledged for Cryo-TEM images of magnetoliposomes and Dr C. Innocenti for scientific discussion. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr Tore Eriksson is acknowledged for building the alternating magnetic field generator. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2011

Y1 - 2011

N2 - The paper describes the effect of a low-frequency alternating magnetic field (LF-AMF) on the permeability and release properties of large (LUVs) and giant (GUVs) unilamellar vesicles loaded with citrate coated cobalt ferrite nanoparticles (NPs). The citrate shell allows a high loading of NPs in lipid vesicles without modifying their magnetic properties. The increase of magnetic LUVs permeability upon exposure to LF-AMF has been evaluated as the fluorescence self-quenching of carboxyfluorescein (CF) entrapped inside the liposome aqueous pool. Liposome leakage has been monitored as a function of field frequency, time exposure and concentration of the citrate coated NPs. Confocal Laser Scanning Microscopy (CLSM) experiments performed on magnetic GUVs labeled with the fluorescent probe DiIC18 and loaded with Alexa 488-C5-maleimide fluorescent dye provided insights on the release mechanism induced by LF-AMF. The results show that LF-AMF strongly affects vesicles permeability, suggesting the formation of pores in the lipid bilayer due to both hyperthermic effects and nanoparticle oscillations in the vesicles pool at the applied frequency. The behaviour of these magnetic vesicles in the presence of LF-AMF makes this system a good candidate for controlled drug delivery. © 2011 The Royal Society of Chemistry.

AB - The paper describes the effect of a low-frequency alternating magnetic field (LF-AMF) on the permeability and release properties of large (LUVs) and giant (GUVs) unilamellar vesicles loaded with citrate coated cobalt ferrite nanoparticles (NPs). The citrate shell allows a high loading of NPs in lipid vesicles without modifying their magnetic properties. The increase of magnetic LUVs permeability upon exposure to LF-AMF has been evaluated as the fluorescence self-quenching of carboxyfluorescein (CF) entrapped inside the liposome aqueous pool. Liposome leakage has been monitored as a function of field frequency, time exposure and concentration of the citrate coated NPs. Confocal Laser Scanning Microscopy (CLSM) experiments performed on magnetic GUVs labeled with the fluorescent probe DiIC18 and loaded with Alexa 488-C5-maleimide fluorescent dye provided insights on the release mechanism induced by LF-AMF. The results show that LF-AMF strongly affects vesicles permeability, suggesting the formation of pores in the lipid bilayer due to both hyperthermic effects and nanoparticle oscillations in the vesicles pool at the applied frequency. The behaviour of these magnetic vesicles in the presence of LF-AMF makes this system a good candidate for controlled drug delivery. © 2011 The Royal Society of Chemistry.

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UR - http://www.scopus.com/inward/record.url?scp=79251523841&partnerID=8YFLogxK

U2 - 10.1039/c0sm00789g

DO - 10.1039/c0sm00789g

M3 - Article

SN - 1744-683X

VL - 7

SP - 1025

EP - 1037

JO - Soft Matter

JF - Soft Matter

IS - 3

ER -

Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability

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