Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads

Christoph Gertler; Rafael Bargiela; Francesca Mapelli; Xifang Han; Jianwei Chen; Tran Hai; Ranya A. Amer; Mouna Mahjoubi; Hanan Issa Malkawi; Mirko Magagnini; Ameur Cherif; Yasser Refaat Abdel-Fattah; Nicolas E. Kalogerakis; Daniele Daffonchio; Manuel Ferrer; Peter N. Golyshin

doi:10.1007/s00248-015-0606-7

Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads

Christoph Gertler, Rafael Bargiela, Francesca Mapelli, Xifang Han, Jianwei Chen, Tran Hai, Ranya A. Amer, Mouna Mahjoubi, Hanan Issa Malkawi, Mirko Magagnini, Ameur Cherif, Yasser Refaat Abdel-Fattah, Nicolas E. Kalogerakis, Daniele Daffonchio, Manuel Ferrer, Peter N. Golyshin

Research output: Contribution to journal › Article › peer-review

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Abstract

Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria. © 2015 Springer Science+Business Media New York

Original language	English (US)
Pages (from-to)	724-740
Number of pages	17
Journal	Microbial Ecology
Volume	70
Issue number	3
DOIs	https://doi.org/10.1007/s00248-015-0606-7
State	Published - Apr 29 2015

ASJC Scopus subject areas

Ecology
Ecology, Evolution, Behavior and Systematics
Soil Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00248-015-0606-7

https://repository.kaust.edu.sa/bitstream/10754/565965/1/10.1007_s00248-015-0606-7%20Micr%20Ecol.pdf

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Gertler, C., Bargiela, R., Mapelli, F., Han, X., Chen, J., Hai, T., Amer, R. A., Mahjoubi, M., Malkawi, H. I., Magagnini, M., Cherif, A., Abdel-Fattah, Y. R., Kalogerakis, N. E., Daffonchio, D., Ferrer, M., & Golyshin, P. N. (2015). Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads. Microbial Ecology, 70(3), 724-740. https://doi.org/10.1007/s00248-015-0606-7

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title = "Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads",

abstract = "Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria. {\textcopyright} 2015 Springer Science+Business Media New York",

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Gertler, C, Bargiela, R, Mapelli, F, Han, X, Chen, J, Hai, T, Amer, RA, Mahjoubi, M, Malkawi, HI, Magagnini, M, Cherif, A, Abdel-Fattah, YR, Kalogerakis, NE, Daffonchio, D, Ferrer, M & Golyshin, PN 2015, 'Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads', Microbial Ecology, vol. 70, no. 3, pp. 724-740. https://doi.org/10.1007/s00248-015-0606-7

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AU - Bargiela, Rafael

AU - Mapelli, Francesca

AU - Han, Xifang

AU - Chen, Jianwei

AU - Hai, Tran

AU - Amer, Ranya A.

AU - Mahjoubi, Mouna

AU - Malkawi, Hanan Issa

AU - Magagnini, Mirko

AU - Cherif, Ameur

AU - Abdel-Fattah, Yasser Refaat

AU - Kalogerakis, Nicolas E.

AU - Daffonchio, Daniele

AU - Ferrer, Manuel

AU - Golyshin, Peter N.

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PY - 2015/4/29

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N2 - Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria. © 2015 Springer Science+Business Media New York

AB - Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria. © 2015 Springer Science+Business Media New York

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ER -

Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads

Abstract

ASJC Scopus subject areas

UN SDGs

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