TY - JOUR
T1 - Copper effects on biomarkers associated with photosynthesis, oxidative status and calcification in the Brazilian coral Mussismilia harttii (Scleractinia, Mussidae)
AU - Marangoni, Laura Fernandes de Barros
AU - Marques, Joseane Aparecida
AU - Duarte, Gustavo Adolpho Santos
AU - Pereira, Cristiano Macedo
AU - Calderon, Emiliano Nicolas
AU - Castro, Clovis Barreira e.
AU - Bianchini, Adalto
N1 - Funding Information:
The International Development Research Centre (IDRC, Ottawa, Canada; grant # 104519-003), Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES ? Programa Ci?ncias do Mar, Bras?lia, DF, Brazil; grant # 84/2010) and Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq ? Instituto Nacional de Ci?ncia e Tecnologia de Toxicologia Aqu?tica, Bras?lia, DF, Brazil; grant # 573949/2008-5) are acknowledged for their financial support. The Coral Vivo Project and its sponsors Petrobras, through the Petrobras Socio-environmental Program, and Arraial d'Ajuda Eco Parque are acknowledged for their support in field research. A. Bianchini is a research fellow from the Brazilian CNPq (Proc. # 307647/2016-1) and supported by the International Canada Research Chair Program from IDRC. L.F.B. Marangoni was a graduate fellow from CAPES.
Funding Information:
The International Development Research Centre (IDRC, Ottawa, Canada; grant # 104519-003), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES – Programa Ciências do Mar, Brasília, DF, Brazil; grant # 84/2010) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq – Instituto Nacional de Ciência e Tecnologia de Toxicologia Aquática, Brasília, DF, Brazil; grant # 573949/2008-5) are acknowledged for their financial support. The Coral Vivo Project and its sponsors Petrobras, through the Petrobras Socio-environmental Program, and Arraial d'Ajuda Eco Parque are acknowledged for their support in field research. A. Bianchini is a research fellow from the Brazilian CNPq (Proc. # 307647/2016-1) and supported by the International Canada Research Chair Program from IDRC. L.F.B. Marangoni was a graduate fellow from CAPES.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017
Y1 - 2017
N2 - Seawater contamination with metals, such as copper (Cu), is a notable local impact threatening coral reefs. Cu effects on biomarkers associated with photosynthesis, oxidative status and calcification were evaluated in the Brazilian coral Mussismilia harttii using a marine mesocosm facility. Polyps were kept under control conditions (1.9 μg L−1 Cu) or exposed to dissolved Cu (3.0, 4.8, and 6.7 μg L−1) for 12 days. Photochemical efficiency of the photosystem II of symbiotic algae (zooxanthellae) was measured and polyps were analyzed for antioxidant capacity, lipid peroxidation, DNA damage, and carbonic anhydrase Ca-ATPase, Mg-ATPase and (Ca,Mg)-ATPase activities after 12 days. Results highlighted the effects of Cu exposure, leading corals to an oxidative stress condition [increased total antioxidant capacity (TAC) and DNA damage] and a possible reduced calcification ability [decreased (Ca,Mg)-ATPase activity]. Therefore, biomarkers associated with oxidative status (TAC and DNA damage) and calcification [(Ca, Mg)-ATPase] are indicated as good predictors of corals health.
AB - Seawater contamination with metals, such as copper (Cu), is a notable local impact threatening coral reefs. Cu effects on biomarkers associated with photosynthesis, oxidative status and calcification were evaluated in the Brazilian coral Mussismilia harttii using a marine mesocosm facility. Polyps were kept under control conditions (1.9 μg L−1 Cu) or exposed to dissolved Cu (3.0, 4.8, and 6.7 μg L−1) for 12 days. Photochemical efficiency of the photosystem II of symbiotic algae (zooxanthellae) was measured and polyps were analyzed for antioxidant capacity, lipid peroxidation, DNA damage, and carbonic anhydrase Ca-ATPase, Mg-ATPase and (Ca,Mg)-ATPase activities after 12 days. Results highlighted the effects of Cu exposure, leading corals to an oxidative stress condition [increased total antioxidant capacity (TAC) and DNA damage] and a possible reduced calcification ability [decreased (Ca,Mg)-ATPase activity]. Therefore, biomarkers associated with oxidative status (TAC and DNA damage) and calcification [(Ca, Mg)-ATPase] are indicated as good predictors of corals health.
KW - Biomarkers
KW - Calcification
KW - Copper
KW - Coral
KW - Ecotoxicology
KW - Marine mesocosm
KW - Mussismilia harttii
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=85022069861&partnerID=8YFLogxK
U2 - 10.1016/j.marenvres.2017.08.002
DO - 10.1016/j.marenvres.2017.08.002
M3 - Article
C2 - 28823595
AN - SCOPUS:85022069861
SN - 0141-1136
VL - 130
SP - 248
EP - 257
JO - Marine environmental research
JF - Marine environmental research
ER -
